Effects of sevofiurane on the cAMP-induced short-circuit current in mouse tracheal epithelium and recombinant Cl- (CFTR) and K+ (KCNQ1) channels
DC Field | Value | Language |
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dc.contributor.author | Kim, J. K. | - |
dc.contributor.author | Yoo, Hae Young | - |
dc.contributor.author | Kim, S. J. | - |
dc.contributor.author | Hwang, Y.-S. | - |
dc.contributor.author | Han, J. | - |
dc.contributor.author | Kim, J. A. | - |
dc.contributor.author | Kim, C. S. | - |
dc.contributor.author | Cho, H. S. | - |
dc.date.accessioned | 2022-02-24T05:41:32Z | - |
dc.date.available | 2022-02-24T05:41:32Z | - |
dc.date.issued | 2007-08 | - |
dc.identifier.issn | 0007-0912 | - |
dc.identifier.issn | 1471-6771 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/55298 | - |
dc.description.abstract | Background. An optimal level of airway surface liquid is essential for mucociliary clearance in lungs. The cAMP-activated cystic fibrosis transmembrane conductance regulator (CFTR) and KCNQ I channels in tracheal epithelium play key roles in luminal and basolateral membranes, respectively. The aim of this study was to examine the effects of sevoflurane on cAMP-induced chloride secretion by the mouse tracheal epithelium and the modulation of recombinant CFTR and KCNQ I channels. Methods. The equivalent short-circuit current (I-sc) of the mouse tracheal epithelium was measured using a flow-type Ussing chamber technique. Inhibition of Na+ absorption was achieved through the luminal application of amiloride. cAMP-dependent CI- secretion was evoked by forskolin and isobutylmethylxanthine (Fsk/IBMX) applied to the basolateral side. The effect of sevoflurane on CFTR and KCNQ I channels was assessed using a whole-cell patch clamp in human embryonic kidney 293T cells expressing CFTR and KCNQ I channels. Results. Fsk/IBMX induced a sustained I-sc that was suppressed by the application of sevoflurane [decreased by 49 (4.5)% at 190 mu M]. The Fsk/IBMX-induced I-sc was also blocked by basolateral application of chromanol 29313, a blocker of the KCNQ I K+ channel. In KCNQ I-expressing cells, sevoflurane 190 mu M reduced the outward currents to 59 (4.9)% at 80 mV The CFTR current was not affected by sevoflurane (similar to 360 mu M). Conclusions. These results suggest that the inhibition of KCNQ I underlies sevoflurane-induced decrease in airway secretion. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | Effects of sevofiurane on the cAMP-induced short-circuit current in mouse tracheal epithelium and recombinant Cl- (CFTR) and K+ (KCNQ1) channels | - |
dc.type | Article | - |
dc.identifier.doi | 10.1093/bja/aem123 | - |
dc.identifier.bibliographicCitation | BRITISH JOURNAL OF ANAESTHESIA, v.99, no.2, pp 245 - 251 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000248683000015 | - |
dc.citation.endPage | 251 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 245 | - |
dc.citation.title | BRITISH JOURNAL OF ANAESTHESIA | - |
dc.citation.volume | 99 | - |
dc.type.docType | Article; Proceedings Paper | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | anaesthetic volatile, sevoflurane | - |
dc.subject.keywordAuthor | ions, ion channels | - |
dc.subject.keywordAuthor | lung, trachea | - |
dc.subject.keywordPlus | AIRWAY SURFACE LIQUID | - |
dc.subject.keywordPlus | VOLATILE ANESTHETICS | - |
dc.subject.keywordPlus | ION-TRANSPORT | - |
dc.subject.keywordPlus | HALOTHANE | - |
dc.subject.keywordPlus | SEVOFLURANE | - |
dc.subject.keywordPlus | ISOFLURANE | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | INHALATION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.relation.journalResearchArea | Anesthesiology | - |
dc.relation.journalWebOfScienceCategory | Anesthesiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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