Charge Storage in Redox-active Azurin Monolayer on 11-MUA Modified Gold Surface

Abstract

The charge storage in the protein-based biomemory consisting of P. aeruginosa azurin was investigated. Azurin was used as an electron donor or acceptor. To immobilize the azurin on the Au surface, 11-mercaptoundecanoic acid was used as a linker material. The azurin-immobilized biofilm on Au surface was optimized by surface plasmon resonance spectroscopy and the surface morphology of protein-based biofilm on Au surface was investigated by atomic force microscope. The memory device characteristics, including the "write", "read" fuctions of the self-assembled azurin biofilm, were well demonstrated with two distinct electrical states of protein-based biofilms by cyclic voltammetry. From the results, it could be concluded that the azurin biofilm could be used for the construction of nanobiochip with memory function.