Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
DC Field | Value | Language |
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dc.contributor.author | Lee ,Jaejin | - |
dc.contributor.author | Shin, Eunkyoung | - |
dc.contributor.author | Yeom, Ji-Hyun | - |
dc.contributor.author | Park, Jaeyoung | - |
dc.contributor.author | Kim, Sunwoo | - |
dc.contributor.author | Lee, Minho | - |
dc.contributor.author | Lee, Kangseok | - |
dc.date.accessioned | 2022-04-11T07:40:14Z | - |
dc.date.available | 2022-04-11T07:40:14Z | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 0882-4010 | - |
dc.identifier.issn | 1096-1208 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/56085 | - |
dc.description.abstract | RNase E-mediated RNA processing and degradation are involved in bacterial adaptation to environmental changes. The RraA regulatory protein, which is highly conserved in γ-proteobacteria, differentially modulates RNase E activity. Recent studies have revealed the association of Salmonella enterica serovar Typhimurium RNase E (STRNase E) with bacterial pathogenicity; however, the molecular mechanisms are unknown. Here, we show that the expression levels of STRraA, a protein regulator of STRNase E activity, affect S. Typhimurium pathogenicity. RNA-sequencing and RT-PCR analyses indicated positive effects of STRraA levels on the abundance of mRNA species from class II flagellar operons. Primer extension analysis further identified STRraA-regulated STRNase E cleavage in the 5′ untranslated region of fliDST mRNA. The cleavage affected the stability of this polycistronic mRNA, suggesting that STRraA protects fliDST mRNA from STRNase E cleavage, leading to enhanced flagellar assembly. Accordingly, STRraA positively regulated flagellar assembly and motility. In addition, STrraA-deleted cells showed decreased invasion ability and cytotoxicity in infection of human cervical epithelial carcinoma cells and reduced mortality in a mouse infection model compared to wild-type cells. These results support an active role of STRraA in RNase E-mediated modulation of pathogenesis in S. Typhimurium. © 2022 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Academic Press | - |
dc.title | Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.micpath.2022.105460 | - |
dc.identifier.bibliographicCitation | Microbial Pathogenesis, v.165 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000793470000005 | - |
dc.identifier.scopusid | 2-s2.0-85126823504 | - |
dc.citation.title | Microbial Pathogenesis | - |
dc.citation.volume | 165 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Pathogenicity | - |
dc.subject.keywordAuthor | RNase E | - |
dc.subject.keywordAuthor | Salmonella Typhimurium | - |
dc.subject.keywordAuthor | STRraA | - |
dc.subject.keywordAuthor | Virulence | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI RRAA | - |
dc.subject.keywordPlus | ENTERICA SEROVAR TYPHIMURIUM | - |
dc.subject.keywordPlus | TYPE-1 FIMBRIAL EXPRESSION | - |
dc.subject.keywordPlus | FLAGELLAR GENE-EXPRESSION | - |
dc.subject.keywordPlus | HOOK-ASSOCIATED PROTEINS | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | III SECRETION | - |
dc.subject.keywordPlus | RIBONUCLEOLYTIC ACTIVITY | - |
dc.subject.keywordPlus | OXYGEN AVAILABILITY | - |
dc.subject.keywordPlus | BACTERIAL FLAGELLUM | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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