Properties of the Hansenula polymorpha-derived constitutive GAP promoter, assessed using an HSA reporter gene
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Heo, Joo-Hyung | - |
dc.contributor.author | Hong, Won Kyoung | - |
dc.contributor.author | Cho, Eun Young | - |
dc.contributor.author | Kim, Moo Woong | - |
dc.contributor.author | Kim, Jeong-Yoon | - |
dc.contributor.author | Kim, Chul Ho | - |
dc.contributor.author | Rhee, Sang Ki | - |
dc.contributor.author | Kang, Hyun Ah | - |
dc.date.accessioned | 2022-04-29T05:40:08Z | - |
dc.date.available | 2022-04-29T05:40:08Z | - |
dc.date.issued | 2003-11 | - |
dc.identifier.issn | 1567-1356 | - |
dc.identifier.issn | 1567-1364 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/56978 | - |
dc.description.abstract | The glyceraldehyde-3-phosphate dehydrogenase promoter, P-GAP, was employed to direct the constitutive expression of recombinant human serum albumin (HSA) in Hansenula polymorpha. A set of integration vectors containing the HSA cDNA under the control of P-GAP was constructed and the elemental parameters affecting the expression of HSA from P-GAP were analyzed. The presence of a 5'-untranslated region derived from the HSA cDNA and the integration of the expression vector into the GAP locus were shown to improve the expression of HSA under P-GAP. Glycerol supported a higher level of HSA expression from P-GAP along with a higher cell density than either glucose or methanol. The growth at high glycerol concentrations up to 12% did not cause any significant repression of the cell growth. A high cell density culture, up to 83 g l(-1) dry cell weight with a HSA production of 550 mg l(-1), was obtained in less than 32 h of cultivation in a fed-batch fermentation employing intermittent feeding with 12% glycerol. The GAP promoter-based HSA expression system showed a higher specific production rate and required a much simpler fermentation process than the MOX promoter-based system, demonstrating that P-GAP can be a practical alternative of the MOX promoter in the large-scale production of HSA from H. polymorpha. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Properties of the Hansenula polymorpha-derived constitutive GAP promoter, assessed using an HSA reporter gene | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/S1567-1356(03)00150-8 | - |
dc.identifier.bibliographicCitation | FEMS YEAST RESEARCH, v.4, no.2, pp 175 - 184 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000186795800007 | - |
dc.identifier.scopusid | 2-s2.0-0242655055 | - |
dc.citation.endPage | 184 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 175 | - |
dc.citation.title | FEMS YEAST RESEARCH | - |
dc.citation.volume | 4 | - |
dc.type.docType | Article; Proceedings Paper | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Constitutive expression | - |
dc.subject.keywordAuthor | Glyceraldehyde-3-phosphate dehydrogenase promoter | - |
dc.subject.keywordAuthor | Hansenula polymorpha | - |
dc.subject.keywordAuthor | High cell density culture | - |
dc.subject.keywordAuthor | Human serum albumin | - |
dc.subject.keywordPlus | HUMAN-SERUM-ALBUMIN | - |
dc.subject.keywordPlus | YEAST SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | PICHIA-PASTORIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DEHYDROGENASE | - |
dc.subject.keywordPlus | VECTORS | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | SEQUENCES | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | GROWTH | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalResearchArea | Mycology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Mycology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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