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Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans

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dc.contributor.authorSong, Yunkyoung-
dc.contributor.authorChoi, Min Hee-
dc.contributor.authorPark, Jeong-Nam-
dc.contributor.authorKim, Moo Woong-
dc.contributor.authorKim, Eun Jung-
dc.contributor.authorKang, Hyun Ah-
dc.contributor.authorKim, Jeong-Yoon-
dc.date.accessioned2022-04-29T07:40:09Z-
dc.date.available2022-04-29T07:40:09Z-
dc.date.issued2007-07-
dc.identifier.issn0099-2240-
dc.identifier.issn1098-5336-
dc.identifier.urihttps://scholarworks.bwise.kr/cau/handle/2019.sw.cau/56994-
dc.description.abstractIn an attempt to engineer a Yarrowia lipolytica strain to produce glycoproteins lacking the outer-chain mannose residues of N-linked oligosaccharides, we investigated the functions of the OCH1 gene encoding a putative alpha-1,6-mannosyltransferase in Y. lipolytica. The complementation of the Saccharomyces cerevisiae och1 mutation by the expression of YlOCH1 and the lack of in vitro alpha-1,6-mannosyltransferase activity in the Yloch1 null mutant indicated that Yl0CH1 is a functional ortholog of S. cerevisiae OCH1. The oligosaccharides assembled on two secretory glycoproteins, the Trichoderma reesei endoglucanase I and the endogenous Y. lipolytica lipase, from the Yloch1 null mutant contained a single predominant species, the core oligosaccharide Man(8)GlcNAc(2), whereas those from the wild-type strain consisted of oligosaccharides with heterogeneous sizes, Man(8)GlcNAc(2) to Man(12)GlcNAc(2). Digestion with alpha-1,2- and alpha-1,6-mannosidase of the oligosaccharides from the wild-type and Yloch1 mutant strains strongly supported the possibility that the Yloch1 mutant strain has a defect in adding the first alpha-1,6-linked mannose to the core oligosaccharide. Taken together, these results indicate that YlOCH1 plays a key role in the outer-chain mannosylation of N-linked oligosaccharides in Y. lipolytica. Therefore, the Yloch1 mutant strain can be used as a host to produce glycoproteins lacking the outer-chain mannoses and further developed for the production of therapeutic glycoproteins containing human-compatible oligosaccharides.-
dc.format.extent9-
dc.language영어-
dc.language.isoENG-
dc.publisherAMER SOC MICROBIOLOGY-
dc.titleEngineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans-
dc.typeArticle-
dc.identifier.doi10.1128/AEM.02058-06-
dc.identifier.bibliographicCitationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.73, no.14, pp 4446 - 4454-
dc.description.isOpenAccessY-
dc.identifier.wosid000248197400007-
dc.identifier.scopusid2-s2.0-34547178785-
dc.citation.endPage4454-
dc.citation.number14-
dc.citation.startPage4446-
dc.citation.titleAPPLIED AND ENVIRONMENTAL MICROBIOLOGY-
dc.citation.volume73-
dc.type.docTypeArticle-
dc.publisher.location미국-
dc.subject.keywordPlusSACCHAROMYCES-CEREVISIAE-
dc.subject.keywordPlusGLYCOSYLATION-
dc.subject.keywordPlusMUTANTS-
dc.subject.keywordPlusELONGATION-
dc.subject.keywordPlusGENE-
dc.subject.keywordPlusALPHA-1,6-MANNOSYLTRANSFERASE-
dc.subject.keywordPlusOLIGOSACCHARIDES-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusAMPLIFICATION-
dc.subject.keywordPlusPROTEINS-
dc.relation.journalResearchAreaBiotechnology & Applied Microbiology-
dc.relation.journalResearchAreaMicrobiology-
dc.relation.journalWebOfScienceCategoryBiotechnology & Applied Microbiology-
dc.relation.journalWebOfScienceCategoryMicrobiology-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
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