Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans
DC Field | Value | Language |
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dc.contributor.author | Song, Yunkyoung | - |
dc.contributor.author | Choi, Min Hee | - |
dc.contributor.author | Park, Jeong-Nam | - |
dc.contributor.author | Kim, Moo Woong | - |
dc.contributor.author | Kim, Eun Jung | - |
dc.contributor.author | Kang, Hyun Ah | - |
dc.contributor.author | Kim, Jeong-Yoon | - |
dc.date.accessioned | 2022-04-29T07:40:09Z | - |
dc.date.available | 2022-04-29T07:40:09Z | - |
dc.date.issued | 2007-07 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.issn | 1098-5336 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/56994 | - |
dc.description.abstract | In an attempt to engineer a Yarrowia lipolytica strain to produce glycoproteins lacking the outer-chain mannose residues of N-linked oligosaccharides, we investigated the functions of the OCH1 gene encoding a putative alpha-1,6-mannosyltransferase in Y. lipolytica. The complementation of the Saccharomyces cerevisiae och1 mutation by the expression of YlOCH1 and the lack of in vitro alpha-1,6-mannosyltransferase activity in the Yloch1 null mutant indicated that Yl0CH1 is a functional ortholog of S. cerevisiae OCH1. The oligosaccharides assembled on two secretory glycoproteins, the Trichoderma reesei endoglucanase I and the endogenous Y. lipolytica lipase, from the Yloch1 null mutant contained a single predominant species, the core oligosaccharide Man(8)GlcNAc(2), whereas those from the wild-type strain consisted of oligosaccharides with heterogeneous sizes, Man(8)GlcNAc(2) to Man(12)GlcNAc(2). Digestion with alpha-1,2- and alpha-1,6-mannosidase of the oligosaccharides from the wild-type and Yloch1 mutant strains strongly supported the possibility that the Yloch1 mutant strain has a defect in adding the first alpha-1,6-linked mannose to the core oligosaccharide. Taken together, these results indicate that YlOCH1 plays a key role in the outer-chain mannosylation of N-linked oligosaccharides in Y. lipolytica. Therefore, the Yloch1 mutant strain can be used as a host to produce glycoproteins lacking the outer-chain mannoses and further developed for the production of therapeutic glycoproteins containing human-compatible oligosaccharides. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans | - |
dc.type | Article | - |
dc.identifier.doi | 10.1128/AEM.02058-06 | - |
dc.identifier.bibliographicCitation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.73, no.14, pp 4446 - 4454 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000248197400007 | - |
dc.identifier.scopusid | 2-s2.0-34547178785 | - |
dc.citation.endPage | 4454 | - |
dc.citation.number | 14 | - |
dc.citation.startPage | 4446 | - |
dc.citation.title | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.citation.volume | 73 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | MUTANTS | - |
dc.subject.keywordPlus | ELONGATION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | ALPHA-1,6-MANNOSYLTRANSFERASE | - |
dc.subject.keywordPlus | OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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