Expression and secretion of human serum albumin in the yeast Saccharomyces cerevisiae
DC Field | Value | Language |
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dc.contributor.author | Kang, Hyun Ah | - |
dc.contributor.author | Jung, Moon Soo | - |
dc.contributor.author | Hong, Won-Kyoung | - |
dc.contributor.author | Sohn, Jung-Hoon | - |
dc.contributor.author | Choi, Eui-Sung | - |
dc.contributor.author | Rhee, Sang-Ki | - |
dc.date.accessioned | 2022-04-29T07:40:16Z | - |
dc.date.available | 2022-04-29T07:40:16Z | - |
dc.date.issued | 1998-02 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57002 | - |
dc.description.abstract | In order to maximize the secretory expression of human serum albumin (HSA) in the yeast Saccharomyces cerevisiae, a series of HSA expression vectors were constructed with a combination of different promoters, 5' untranslated regions (5'UTR), and secretion signal sequences. The expression vector composed of the galactose-inducible promoter GAL10, the natural 5'UTR, and the natural signal sequence of HSA directed the most efficient expression and secretion of HSA vectors introduced into when Although the major form of HSA expressed and secreted in the yeast transformants was the mature form of 66 kDa, the truncated form of 45 kDa was also detected both in the cell extract and in the culture supernatant. The level of the intact HSA protein in the culture supernatant reached up to 30 mg/l at 24 h of cultivation in a shake-flask culture but began to decrease afterwards, indicating that the secreted HSA protein was unstable in a prolonged culture of yeast. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.title | Expression and secretion of human serum albumin in the yeast Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.8, no.1, pp 42 - 48 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000072967400007 | - |
dc.identifier.scopusid | 2-s2.0-6844266270 | - |
dc.citation.endPage | 48 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 42 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 8 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Human serum albumin | - |
dc.subject.keywordAuthor | Saccharomyces cerevisiae | - |
dc.subject.keywordAuthor | Secretion | - |
dc.subject.keywordPlus | HIGH-LEVEL SECRETION | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | 2-MU-M CIRCLE | - |
dc.subject.keywordPlus | COPY NUMBER | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | GALACTOSE | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | VECTORS | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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