Coexpression of BiP increased antithrombotic hirudin production in recombinant Sacchdromyces cerevisiae
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Myoung-Dong | - |
dc.contributor.author | Han, Ki-Cheol | - |
dc.contributor.author | Kang, Hyun-Ah | - |
dc.contributor.author | Rhee, Sang-Ki | - |
dc.contributor.author | Seo, Jin-Ho | - |
dc.date.accessioned | 2022-04-29T08:40:13Z | - |
dc.date.available | 2022-04-29T08:40:13Z | - |
dc.date.issued | 2003-02 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.issn | 1873-4863 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57010 | - |
dc.description.abstract | In order to increase a production level of antithrombotic hirudin, BiP was simultaneously expressed in recombinant Saccharomyces cerevisiae strains carrying ten and 15 copies of the hirudin expression cassette integrated in the chromosome. Coexpression of BiP greatly enhanced both cell growth and hirudin production in recombinant S. cerevisiae. Maximum hirudin concentration of 36 mg1(-1) was obtained from batch culture of the ten copy-number transformant concomitantly harboring an episomal copy of the BiP gene under the control of the GAL promoter, which is corresponding to a 2.5-fold increase compared with the control strain carrying the genomic BiP gene only. The mean size of the recombinant yeast cells expressing the BiP gene remained at a relatively constant level compared with the control strains of which size increased after the onset of hirudin expression by the GAL10 promoter. (C) 2002 Elsevier Science B.V. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Coexpression of BiP increased antithrombotic hirudin production in recombinant Sacchdromyces cerevisiae | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/S0168-1656(02)00288-2 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOTECHNOLOGY, v.101, no.1, pp 81 - 87 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000180677700009 | - |
dc.identifier.scopusid | 2-s2.0-0037468244 | - |
dc.citation.endPage | 87 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 81 | - |
dc.citation.title | JOURNAL OF BIOTECHNOLOGY | - |
dc.citation.volume | 101 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | BiP | - |
dc.subject.keywordAuthor | GAL10 promoter | - |
dc.subject.keywordAuthor | Hirudin | - |
dc.subject.keywordAuthor | Saccharomyces cerevisiae | - |
dc.subject.keywordAuthor | Secretion | - |
dc.subject.keywordPlus | HETEROLOGOUS PROTEIN SECRETION | - |
dc.subject.keywordPlus | YEAST ENDOPLASMIC-RETICULUM | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | ANTICOAGULANT HIRUDIN | - |
dc.subject.keywordPlus | SELECTIVE PROTEINS | - |
dc.subject.keywordPlus | BINDING-PROTEIN | - |
dc.subject.keywordPlus | CHAPERONE BIP | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.