Biochemical Characterization of a Glycosyltransferase Homolog from an Oral Pathogen Fusobacterium nucleatum as a Human Glycan-modifying Enzyme
DC Field | Value | Language |
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dc.contributor.author | Kim, Seonghun | - |
dc.contributor.author | Oh, Doo-Byoung | - |
dc.contributor.author | Kwon, Ohsuk | - |
dc.contributor.author | Jung, Jae Kap | - |
dc.contributor.author | Lee, Yun Mi | - |
dc.contributor.author | Ko, Kisung | - |
dc.contributor.author | Ko, Jeong Heon | - |
dc.contributor.author | Kang, Hyun Ah | - |
dc.date.accessioned | 2022-05-11T02:40:30Z | - |
dc.date.available | 2022-05-11T02:40:30Z | - |
dc.date.issued | 2008-05 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57516 | - |
dc.description.abstract | Bacterial glycosyltransferases have drawn growing attention as economical enzymes for oligosaccharide synthesis, with their easy expression and relatively broad substrate specificity. Here, we characterized a glycosyltransferase homolog (Fnu_GT) from a human oral pathogen, Fusobacterium nucleatum. Bioinformatic analysis showed that Fnu_GT belongs to the glycosyltransferases family II. The recombinant Fnu_GT (rFnu_GT) expressed in Escherichia coli displayed the highest glycosylation activity when UDP-galactose (Gal) was used as a donor nucleotide-sugar with heptose or N-acetylglucosamine (GlcNAc) as an acceptor sugar. Interestingly, rFnu_GT transferred the galactose moiety of UDP-Gal to a nonreducing terminal GlcNAc attached to the trimannosyl core glycan, indicating its potential as an enzyme for human-type N-glycan synthesis. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | Biochemical Characterization of a Glycosyltransferase Homolog from an Oral Pathogen Fusobacterium nucleatum as a Human Glycan-modifying Enzyme | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, v.18, no.5, pp 859 - 865 | - |
dc.identifier.kciid | ART001260187 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000256238100008 | - |
dc.identifier.scopusid | 2-s2.0-50249164691 | - |
dc.citation.endPage | 865 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 859 | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.volume | 18 | - |
dc.identifier.url | https://www.jmb.or.kr/journal/view.html?uid=2521&vmd=Full | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Glycosyltransferase | - |
dc.subject.keywordAuthor | nucleotide-sugar | - |
dc.subject.keywordAuthor | Fusobacterium nucleatum | - |
dc.subject.keywordAuthor | human-type N-linked glycan | - |
dc.subject.keywordPlus | CAPSULAR POLYSACCHARIDEHAEMOPHILUS-INFLUENZAEESCHERICHIA-COLIMOLECULAR CHARACTERIZATIONNEISSERIA-MENINGITIDISSTRAINLIPOOLIGOSACCHARIDEIDENTIFICATIONLIPOPOLYSACCHARIDESEXPRESSION | - |
dc.relation.journalResearchArea | Biotechnology & Applied MicrobiologyMicrobiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied MicrobiologyMicrobiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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