Impeller types and feeding modes influence the morphology and protein expression in the submerged culture of Aspergillus oryzae
DC Field | Value | Language |
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dc.contributor.author | Heo, Joo-Hyung | - |
dc.contributor.author | Ananin, Vladimir | - |
dc.contributor.author | Park, Jeong-Seok | - |
dc.contributor.author | Lee, Chung-Ryul | - |
dc.contributor.author | Moon, Jun-Ok | - |
dc.contributor.author | Kwon, Ohsuk | - |
dc.contributor.author | Kang, Hyun-Ah | - |
dc.contributor.author | Kim, Chul Ho | - |
dc.contributor.author | Rhee, Sang Ki | - |
dc.date.accessioned | 2022-05-11T02:40:34Z | - |
dc.date.available | 2022-05-11T02:40:34Z | - |
dc.date.issued | 2004-06 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.issn | 1976-3816 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57520 | - |
dc.description.abstract | The influences of impeller types on morphology and protein expression were investigated in a submerged culture of Aspergillus oryzae. The impeller types strongly affected mycelial morphology and protein production in batch and fed-batch fermentations. Cells that were cultured by propeller agitation grew in the form of a pellet, whereas cells that were cultured by turbine agitation grew in a freely dispersed-hyphal manner and in a clumped form. Pellet-grown cells showed high levels of protein production for both the intracellularly heterologous protein (β-glucuronidase) and the extracellularly homologous protein (α-amylase). The feeding mode of the carbon source also influenced the morphological distribution and protein expression in fed-batch fermentation of A. oryzae. Pulsed-feeding mainly showed high protein expression and homogeneous distribution of pellet whereas continuous feeding resulted in less protein expression and heterogeneous distribution with pellet and dispersed-hyphae. The pellet growth with propeller agitation paralleling with the pulsed-feeding of carbon source showed a high level of protein production in the submerged fed-batch fermentation of recombinant A. oryzae. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Korean Society for Biotechnology and Bioengineering | - |
dc.title | Impeller types and feeding modes influence the morphology and protein expression in the submerged culture of Aspergillus oryzae | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/BF02942290 | - |
dc.identifier.bibliographicCitation | Biotechnology and Bioprocess Engineering, v.9, no.3, pp 184 - 190 | - |
dc.identifier.kciid | ART001140617 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000222419500007 | - |
dc.identifier.scopusid | 2-s2.0-30344440454 | - |
dc.citation.endPage | 190 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 184 | - |
dc.citation.title | Biotechnology and Bioprocess Engineering | - |
dc.citation.volume | 9 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Aspergillus oryzae | - |
dc.subject.keywordAuthor | Fed-batch fermentation | - |
dc.subject.keywordAuthor | Filamentous fungi | - |
dc.subject.keywordAuthor | Morphology | - |
dc.subject.keywordAuthor | Propeller agitation | - |
dc.subject.keywordAuthor | Submerged culture | - |
dc.subject.keywordPlus | MYCELIAL MORPHOLOGYFILAMENTOUS FUNGIESCHERICHIA-COLIFERMENTATIONGROWTHGENEGLUCURONIDASEAGITATIONVISCOSITYDELETION | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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