Secretion of active urokinase-type plasminogen activator from the yeast Yarrowia lipolytica
DC Field | Value | Language |
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dc.contributor.author | Ryu, Ho Myoung | - |
dc.contributor.author | Kang, Woo Kyu | - |
dc.contributor.author | Kang, Hyun Ah | - |
dc.contributor.author | Kim, Jeong-Yoon | - |
dc.date.accessioned | 2022-05-11T02:40:36Z | - |
dc.date.available | 2022-05-11T02:40:36Z | - |
dc.date.issued | 2003-04 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.issn | 1976-3816 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57523 | - |
dc.description.abstract | In order to study the secretion of the human urokinase-type plasminogen activator, u-PA, from the yeast Yarrowia lipolytica, three kinds of integrative expression vector were constructed. These vectors differed only in their secretion control regions, pre-, pre-dip- (dipeptide stretch) or pre-dip-pro sequences of the alkaline extracellular protease, which were joined inframe to the human u-PA cDNA. The recombinant Y. lipolytica strains, transformed with the expression vectors, secreted the hyperglycosylated u-PA. A fibrin plate assay of the culture supernatants showed that the hyperglycosylated u-PA proteins could catalyze fibrinolysis, and that the pre-dip sequence was the most efficient secretory signal for the secretion of the u-PA from Y. lipolytica. This result suggests that Y. lipolytica can be developed as a potential host for the production of recombinant human u-PA. ?KSBB. | - |
dc.format.extent | 4 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Korean Society for Biotechnology and Bioengineering | - |
dc.title | Secretion of active urokinase-type plasminogen activator from the yeast Yarrowia lipolytica | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/BF02940274 | - |
dc.identifier.bibliographicCitation | Biotechnology and Bioprocess Engineering, v.8, no.2, pp 162 - 165 | - |
dc.identifier.kciid | ART001192368 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000188831000019 | - |
dc.identifier.scopusid | 2-s2.0-43749090351 | - |
dc.citation.endPage | 165 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 162 | - |
dc.citation.title | Biotechnology and Bioprocess Engineering | - |
dc.citation.volume | 8 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Secretion | - |
dc.subject.keywordAuthor | Urokinase-type plasminogen activator | - |
dc.subject.keywordAuthor | Yarrowia lipolytica | - |
dc.subject.keywordPlus | GAMMA-DECALACTONE PRODUCTIONSACCHAROMYCES-CEREVISIAEEXPRESSIONGLYCOSYLATIONIMPROVEMENTGENETICSPATHWAYCLONINGHOMOLOGSITE | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kciCandi | - |
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