Non-AUG translational initiation of a short CAPC transcript generating protein isoform
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Anaganti, Suresh | - |
dc.contributor.author | Hansen, Johanna K. | - |
dc.contributor.author | Ha, Duc | - |
dc.contributor.author | Hahn, Yoonsoo | - |
dc.contributor.author | Chertov, Oleg | - |
dc.contributor.author | Pastan, Ira | - |
dc.contributor.author | Bera, Tapan K. | - |
dc.date.accessioned | 2022-05-11T07:40:16Z | - |
dc.date.available | 2022-05-11T07:40:16Z | - |
dc.date.issued | 2009-03 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57569 | - |
dc.description.abstract | CAPC (also known as LRRC26) is a new gene with restricted expression in normal tissues, and with expression in many cancers and cancer cell lines. We have identified and characterized a short-transcript of CAPC(S-CAPC). The nucleotide sequence analysis of CAPC mRNA showed that the transcription for S-CAPC starts at position +610 on the L-CAPC transcript. Interestingly, no translation initiation codon 'AUG' is present in this transcript. To determine if a non-AUG start site is utilized, the S-CAPC sequence was cloned into an expression vector With C-terminal myc and histidine tags, and transfected into 293T cells. Western blot and MALDI-TOF MS analysis on purified S-CAPC gave two distinct peaks at approximately 7.5 kDa. N-terminal amino acid sequencing of the purified 7.5 kDa protein product indicated that translation starts at the codon for cysteine on the S-CAPC transcript generating a 7.5 kDa CAPC Protein products translated from a non-AUG initiation site. Published by Elsevier Inc | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Non-AUG translational initiation of a short CAPC transcript generating protein isoform | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbrc.2009.01.089 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.380, no.3, pp 508 - 513 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000264222400015 | - |
dc.identifier.scopusid | 2-s2.0-60549091503 | - |
dc.citation.endPage | 513 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 508 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 380 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | LRRC26 | - |
dc.subject.keywordAuthor | Prostate cancer | - |
dc.subject.keywordAuthor | Breast cancer | - |
dc.subject.keywordAuthor | Alternate transcript | - |
dc.subject.keywordAuthor | Non-AUG initiation | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | HIGH EXPRESSION | - |
dc.subject.keywordPlus | CODONS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.