Abietic acid alleviates endoplasmic reticulum stress and lipid accumulation in human primary hepatocytes through the AMPK/ORP150 signaling
- Authors
- Jung, Tae Woo; Jeong, Ju Cheol; Park, Seung Yeon; Cho, Wonjun; Oh, Heeseung; Lee, Hyun Jung; Hacimuftuoglu, Ahmet; Abd El-Aty, A.M.; Bang, Joon Seok; Jeong, Ji Hoon
- Issue Date
- Jun-2022
- Publisher
- Elsevier B.V.
- Keywords
- Abietic acid; AMPK; ER stress; NAFLD; ORP150
- Citation
- Biochemical and Biophysical Research Communications, v.608, pp 142 - 148
- Pages
- 7
- Journal Title
- Biochemical and Biophysical Research Communications
- Volume
- 608
- Start Page
- 142
- End Page
- 148
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57719
- DOI
- 10.1016/j.bbrc.2022.04.010
- ISSN
- 0006-291X
1090-2104
- Abstract
- Abietic acid (AA), the main component of pine resin that has been traditionally used as Asian medicine, has been reported to demonstrate anti-inflammatory activities. Despite this, little is known about the effects of AA on hepatic endoplasmic reticulum (ER) stress and lipid metabolism. This study investigated the impacts of AA on ER stress and steatosis in in vitro obesity models. We found that Treatment with AA reduced lipid deposition and lipogenesis-related proteins expression in human primary hepatocytes. Augmented expression of ER stress markers (phospho-eukaryotic initiation factor-2α (eIF2α) and C/EBP homologous protein (CHOP)) in palmitate-treated hepatocytes were reversed by AA treatment. Further, AA treatment increased the expression of phospho-AMPK and oxygen-regulated protein 150 (ORP150) in hepatocytes. siRNA-associated knockdown of AMPK or ORP150 expression reduced the effects of AA on not only hepatic ER stress but also lipogenesis and apoptosis. These results denote that AA attenuates lipid accumulation in hepatocytes in the presence of palmitate through the suppression of ER stress by AMPK/ORP150 signaling. AA could be a potential candidate for treating non-alcoholic fatty liver disease. © 2022 Elsevier Inc.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > College of Medicine > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.