In vitro and in vivo anti-tumor efficacy of krill oil against bladder cancer: Involvement of tumor-associated angiogenic vasculature
DC Field | Value | Language |
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dc.contributor.author | Kim, Hoon | - |
dc.contributor.author | Roh, Youngjin | - |
dc.contributor.author | Park, Sang Yong | - |
dc.contributor.author | Lee, Chungil | - |
dc.contributor.author | Lim, Sujin | - |
dc.contributor.author | Cho, Seongbin | - |
dc.contributor.author | Lee, Hyang-Yeol | - |
dc.contributor.author | Hong, Soon Auck | - |
dc.contributor.author | Lee, Tae Jin | - |
dc.contributor.author | Myung, Soon Chul | - |
dc.contributor.author | Yun, Seok-Joong | - |
dc.contributor.author | Choi, Yung Hyun | - |
dc.contributor.author | Kim, Wun-Jae | - |
dc.contributor.author | Moon, Sung-Kwon | - |
dc.date.accessioned | 2022-05-16T09:40:09Z | - |
dc.date.available | 2022-05-16T09:40:09Z | - |
dc.date.issued | 2022-06 | - |
dc.identifier.issn | 0963-9969 | - |
dc.identifier.issn | 1873-7145 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57723 | - |
dc.description.abstract | Krill oil (KO) obtained from Euphausia superba is nutrient-rich and has a positive effect on human health. Here, we explored the efficacy of KO in inhibiting tumor progression and tumor vascularization. KO (100–300 μg/mL) repressed the proliferation of bladder tumor cell lines MBT-2 and T24. Treatment of cells with KO raised cell-cycle arrest at G0/G1-phase via modulation of positive regulators and negative regulators in bladder cancer cells. KO treatment regulated phosphorylation of proteins involved in PI3K/AKT and MAPK signaling pathways, including ERK, JNK, and p38 MAPK. Additionally, KO hampered the invasion and migration of both cell lines via reduction of MMP-9 expression levels by disrupting transcriptional binding of Sp-1, AP-1, and NF-κB motifs. Moreover, in animal studies, KO (150–300 mg/kg) significantly decreased tumor growth in xenograft mice bearing T24 tumor cells. No significant toxic effects were observed in acute toxicity tests, including biological analysis and H&E staining. The reduced level of CD31 expression in KO-treated tumor tissues prompted us to investigate the effect of KO on tumor angiogenesis. KO (5–40 μg/mL) treatment impeded VEGF-induced capillary tube formation and proliferation by inhibiting VEGFR2-modulated eNOS/AKT/ERK1/2 signaling axis in HUVECs. Treatment of HUVECs with KO inhibited VEGF-stimulated migration and invasion by reducing MMP-2 expression level. VEGF-driven sprouting capacity of neo-microvessels was suppressed in the presence of KO (20–40 μg/mL), as determined via an ex vivo aortic ring assay. Our results indicated that KO can regulate both tumor growth and tumor-associated angiogenesis via a novel mechanism. Thus, KO may be a promising antitumor candidate, potentially useful to prevent or treat bladder cancer. © 2022 Elsevier Ltd | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier Ltd | - |
dc.title | In vitro and in vivo anti-tumor efficacy of krill oil against bladder cancer: Involvement of tumor-associated angiogenic vasculature | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.foodres.2022.111144 | - |
dc.identifier.bibliographicCitation | Food Research International, v.156 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000796627700004 | - |
dc.identifier.scopusid | 2-s2.0-85127585569 | - |
dc.citation.title | Food Research International | - |
dc.citation.volume | 156 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Acute toxicity test | - |
dc.subject.keywordAuthor | Aortic ring | - |
dc.subject.keywordAuthor | Krill oil | - |
dc.subject.keywordAuthor | Tumor-associated angiogenic vasculature | - |
dc.subject.keywordAuthor | Xenograft mice | - |
dc.subject.keywordPlus | POLYUNSATURATED FATTY-ACIDS | - |
dc.subject.keywordPlus | EUPHAUSIA-SUPERBA | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | PI3K/AKT PATHWAY | - |
dc.subject.keywordPlus | TYROSINE KINASE | - |
dc.subject.keywordPlus | GROWTH-FACTORS | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | EXTRACTION | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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