Identification and quantification of sialylated and core-fucosylated N-glycans in human transferrin by UPLC and LC-MS/MS
DC Field | Value | Language |
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dc.contributor.author | Jin, M. | - |
dc.contributor.author | Kim, J. | - |
dc.contributor.author | Ha, J. | - |
dc.contributor.author | Kim, A. | - |
dc.contributor.author | Lee, J. | - |
dc.contributor.author | Park, C.S. | - |
dc.contributor.author | Kang, M. | - |
dc.contributor.author | Kim, J. | - |
dc.contributor.author | Mun, C. | - |
dc.contributor.author | Kim, J. | - |
dc.contributor.author | Kim, H.H. | - |
dc.date.accessioned | 2022-05-18T07:40:28Z | - |
dc.date.available | 2022-05-18T07:40:28Z | - |
dc.date.issued | 2022-06 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.issn | 1096-0309 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/57784 | - |
dc.description.abstract | Sialylated and core-fucosylated N-glycans in human transferrin (HTF) are used as glycan biomarkers due to their increased or decreased characteristics in certain diseases. However, their absolute quantities remain unclear. In this study, N-glycans of HTF were identified by UPLC and LC-MS/MS using fluorescence tags [2-aminobenzamide (AB) and procainamide (ProA)] and columns [HILIC and anion exchange chromatography-HILIC (AXH)]. The structures of 14 (including five core-fucosylated) N-glycans in total comprising two non-, six mono-, four di-, and two tri-sialylated N-glycans were identified. The quantities (%) of each N-glycan relative to the total N-glycans (100%) were obtained. HILIC and AXH were better for peak identification and separability except for desialylation, respectively. Specifically, sialylated (in ProA-HILIC and ProA-AXH by UPLC or LC-MS/MS) and core-fucosylated (in AB-HILIC and ProA-AXH by UPLC) N-glycans were efficiently identified. Seven neuraminidase-treated (including three core-fucosylated) N-glycans were efficiently identified in ProA-AXH, even their poor separation. Additionally, ProA-AXH was more efficient for the estimation of the absolute quantities of N-glycans from the results of fluorescence intensity (by UPLC) and relative quantity (by LC-MS/MS). These results first demonstrate that ProA is useful for identifying and quantifying sialylated, core-fucosylated, and neuraminidase-treated desialylated N-glycans in HTF using AXH by UPLC and LC/MS. © 2022 Elsevier Inc. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Academic Press Inc. | - |
dc.title | Identification and quantification of sialylated and core-fucosylated N-glycans in human transferrin by UPLC and LC-MS/MS | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.ab.2022.114650 | - |
dc.identifier.bibliographicCitation | Analytical Biochemistry, v.647 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000830644500004 | - |
dc.identifier.scopusid | 2-s2.0-85127511786 | - |
dc.citation.title | Analytical Biochemistry | - |
dc.citation.volume | 647 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Column | - |
dc.subject.keywordAuthor | Core-fucosylated N-glycan | - |
dc.subject.keywordAuthor | Fluorescent tag | - |
dc.subject.keywordAuthor | LC-MS/MS | - |
dc.subject.keywordAuthor | Sialylated N-glycan | - |
dc.subject.keywordAuthor | UPLC | - |
dc.subject.keywordPlus | CARBOHYDRATE-DEFICIENT TRANSFERRIN | - |
dc.subject.keywordPlus | CONGENITAL DISORDERS | - |
dc.subject.keywordPlus | ACETYLNEURAMINIC ACID | - |
dc.subject.keywordPlus | SERUM TRANSFERRIN | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | PROCAINAMIDE | - |
dc.subject.keywordPlus | CHROMATOGRAPHY | - |
dc.subject.keywordPlus | MARKER | - |
dc.subject.keywordPlus | HPLC | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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