Induction of apoptotic cell death in human bladder cancer cells by ethanol extract of Zanthoxylum schinifolium leaf, through ROS-dependent inactivation of the PI3K/Akt signaling pathway
DC Field | Value | Language |
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dc.contributor.author | Park, Cheol | - |
dc.contributor.author | Choi, Eun Ok | - |
dc.contributor.author | Hwangbo, Hyun | - |
dc.contributor.author | Lee, Hyesook | - |
dc.contributor.author | Jeong, Jin-Woo | - |
dc.contributor.author | Han, Min Ho | - |
dc.contributor.author | Moon, Sung-Kwon | - |
dc.contributor.author | Yun, Seok Joong | - |
dc.contributor.author | Kim, Wun-Jae | - |
dc.contributor.author | Kim, Gi-Young | - |
dc.contributor.author | Hwang, Hye-Jin | - |
dc.contributor.author | Choi, Yung Hyun | - |
dc.date.accessioned | 2022-06-10T05:41:24Z | - |
dc.date.available | 2022-06-10T05:41:24Z | - |
dc.date.issued | 2022-06 | - |
dc.identifier.issn | 1976-1457 | - |
dc.identifier.issn | 2005-6168 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/58264 | - |
dc.description.abstract | BACKGROUND/OBJECTIVES Zanthoxylum schinifolium is traditionally used as a spice for cooking in East Asian countries. This study was undertaken to evaluate the anti-proliferative potential of ethanol extracts of Z. schinifolium leaves (EEZS) against human bladder cancer T24 cells. MATERIALS/METHODS Subsequent to measuring the cytotoxicity of EEZS, the anti-cancer activity was measured by assessing apoptosis induction, reactive oxygen species (ROS) generation, and mitochondrial membrane potential (MMP). In addition, we determined the underlying mechanism of EEZS-induced apoptosis through various assays, including Western blot analysis. RESULTS EEZS treatment concentration-dependently inhibited T24 cell survival, which is associated with apoptosis induction. Exposure to EEZS induced the expression of Fas and Fas-ligand, activated caspases, and subsequently resulted to cleavage of poly (ADP-ribose) polymerase. EEZS also enhanced the expression of cytochrome c in the cytoplasm by suppressing MMP, following increase in the ratio of Bax:Bcl-2 expression and truncation of Bid. However, EEZS-mediated growth inhibition and apoptosis were significantly diminished by a pan-caspase inhibitor. Moreover, EEZS inhibited activation of the phosphoinositide 3-kinase (PI3K)/Akt pathway, and the apoptosis-inducing potential of EEZS was promoted in the presence of PI3K/Akt inhibitor. In addition, EEZS enhanced the production of ROS, whereas N-acetyl cysteine (NAC), a ROS scavenger, markedly suppressed growth inhibition and inactivation of the PI3K/Akt signaling pathway induced by EEZS. Furthermore, NAC significantly attenuated the EEZS-induced apoptosis and reduction of cell viability. CONCLUSIONS Taken together, our results indicate that exposure to EEZS exhibits anti-cancer activity in T24 bladder cancer cells through ROS-dependent induction of apoptosis and inactivation of the PI3K/Akt signaling pathway. | - |
dc.format.extent | 14 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 한국영양학회 | - |
dc.title | Induction of apoptotic cell death in human bladder cancer cells by ethanol extract of Zanthoxylum schinifolium leaf, through ROS-dependent inactivation of the PI3K/Akt signaling pathway | - |
dc.type | Article | - |
dc.identifier.doi | 10.4162/nrp.2022.16.3.330 | - |
dc.identifier.bibliographicCitation | Nutrition Research and Practice, v.16, no.3, pp 330 - 343 | - |
dc.identifier.kciid | ART002841970 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000805309300004 | - |
dc.identifier.scopusid | 2-s2.0-85131877094 | - |
dc.citation.endPage | 343 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 330 | - |
dc.citation.title | Nutrition Research and Practice | - |
dc.citation.volume | 16 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | caspases | - |
dc.subject.keywordAuthor | mitochondria | - |
dc.subject.keywordAuthor | cytochrome epsilon | - |
dc.subject.keywordPlus | COUMARINS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | LEAVES | - |
dc.relation.journalResearchArea | Nutrition & Dietetics | - |
dc.relation.journalWebOfScienceCategory | Nutrition & Dietetics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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