Square microchannel enables to focus and orient ellipsoidal Euglena gracilis cells by two-dimensional acoustic standing wave
DC Field | Value | Language |
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dc.contributor.author | Park, Sungryul | - |
dc.contributor.author | Lee, Sangwook | - |
dc.contributor.author | Kim, Hyun Soo | - |
dc.contributor.author | Choi, Hong Jin | - |
dc.contributor.author | Jeong, Ok Chan | - |
dc.contributor.author | Lin, Ruixian | - |
dc.contributor.author | Cho, Younghak | - |
dc.contributor.author | Lee, Min Ho | - |
dc.date.accessioned | 2022-10-26T06:40:20Z | - |
dc.date.available | 2022-10-26T06:40:20Z | - |
dc.date.issued | 2022-09 | - |
dc.identifier.issn | 0026-3672 | - |
dc.identifier.issn | 1436-5073 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/58882 | - |
dc.description.abstract | Flow cytometry has become an indispensable tool for counting, analyzing, and sorting large cell populations in biological research and medical practice. Unfortunately, it has limitations in the analysis of non-spherically shaped cells due to the variation of their alignment with respect to the flow direction and, hence, the optical interrogation axis, resulting in unreliable cell analysis. Here, we present a simple on-chip acoustofluidic method to fix the orientation of ellipsoidal cells and focus them into a single, aligned stream. Specifically, by generating acoustic standing waves inside a 100 . 100 mu m square-shaped microchannel, we successfully aligned and focused up to 97.7% of a population of Euglena gracilis (an ellipsoidal shaped microalgal species) cells in the center of the microchannel with high precision at a volume rate of 25 to 200 mu L min(-1). Uniform positioning of ellipsoidal cells is essential for making flow cytometry applicable to the investigation of a greater variety of cell populations and is expected to be beneficial for ecological studies and aquaculture. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER WIEN | - |
dc.title | Square microchannel enables to focus and orient ellipsoidal Euglena gracilis cells by two-dimensional acoustic standing wave | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s00604-022-05439-7 | - |
dc.identifier.bibliographicCitation | MICROCHIMICA ACTA, v.189, no.9 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000840854400003 | - |
dc.identifier.scopusid | 2-s2.0-85135950295 | - |
dc.citation.number | 9 | - |
dc.citation.title | MICROCHIMICA ACTA | - |
dc.citation.volume | 189 | - |
dc.type.docType | Article | - |
dc.publisher.location | 오스트리아 | - |
dc.subject.keywordAuthor | Acoustofluidics | - |
dc.subject.keywordAuthor | Microalgae | - |
dc.subject.keywordAuthor | Acoustic focusing | - |
dc.subject.keywordAuthor | Microfluidics | - |
dc.subject.keywordAuthor | Flow cytometry | - |
dc.subject.keywordPlus | FLOW-CYTOMETRY | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | MICROFLUIDICS | - |
dc.subject.keywordPlus | NANOPLANKTON | - |
dc.subject.keywordPlus | PARTICLES | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | CHIP | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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