Depletion of human micro-RNA miR-125b reveals that it is critical for the proliferation of differentiated cells but not for the down-regulation of putative targets during differentiation
DC Field | Value | Language |
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dc.contributor.author | Lee, Yong Sun | - |
dc.contributor.author | Kim, Hak Kyun | - |
dc.contributor.author | Chung, Sangmi | - |
dc.contributor.author | Kim, Kwang-Soo | - |
dc.contributor.author | Dutta, Anindya | - |
dc.date.accessioned | 2022-10-28T06:40:08Z | - |
dc.date.available | 2022-10-28T06:40:08Z | - |
dc.date.issued | 2005-04 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/58921 | - |
dc.description.abstract | Micro-RNAs are small non-coding RNAs that regulate target gene expression post-transcriptionally through base pairing with the target messenger RNA. Functional characterization of micro-RNAs awaits robust experimental methods to knock-down a micro-RNA as well as to assay its function in vivo. In addition to the recently developed method to sequester micro-RNA with 2'-O-methyl antisense oligonucleotide, we report that small interfering RNA against the loop region of a micro-RNA precursor can be used to deplete the micro-RNA. The depletion of miR-125b by this method had a profound effect on the proliferation of adult differentiated cancer cells, and this proliferation defect was rescued by cotransfected mature micro-RNA. This technique has unique advantages over the 2'-O-methyl antisense oligonucleotide and can be used to determine micro-RNA function, assay micro-RNAs in vivo, and identify the contribution of a predicted micro-RNA precursor to the pool of mature micro-RNA in a given cell. miR-125b and let-7 micro-RNAs are induced, whereas their putative targets, lin-28 and lin-41, are decreased during in vitro differentiation of Tera-2 or embryonic stem cells. Experimental increase or decrease of micro-RNA concentrations did not, however, affect the levels of the targets, a finding that is explained by the fact that the down-regulation of the targets appears to be mostly at the transcriptional level in these in vitro differentiation systems. Collectively these results reveal the importance of micro-RNA depletion strategies for directly determining micro-RNA function in vivo. | - |
dc.format.extent | 7 | - |
dc.publisher | American Society for Biochemistry and Molecular Biology Inc. | - |
dc.title | Depletion of human micro-RNA miR-125b reveals that it is critical for the proliferation of differentiated cells but not for the down-regulation of putative targets during differentiation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1074/jbc.M412247200 | - |
dc.identifier.bibliographicCitation | Journal of Biological Chemistry, v.280, no.17, pp 16635 - 16641 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000228615500014 | - |
dc.identifier.scopusid | 2-s2.0-20444440706 | - |
dc.citation.endPage | 16641 | - |
dc.citation.number | 17 | - |
dc.citation.startPage | 16635 | - |
dc.citation.title | Journal of Biological Chemistry | - |
dc.citation.volume | 280 | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | SMALL TEMPORAL RNASC-ELEGANSINTERFERENCEEXPRESSIONIDENTIFICATIONPROTEINDICERCONSERVATIONSEQUENCELIN-28 | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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