The Kleisin Subunits of Cohesin are Involved in the Fate Determination of Embryonic Stem Cells
DC Field | Value | Language |
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dc.contributor.author | Koh, Young Eun | - |
dc.contributor.author | Choi, Eui-Hwan | - |
dc.contributor.author | Kim, Jung-Woong | - |
dc.contributor.author | Kim, Keun Pil | - |
dc.date.accessioned | 2022-10-31T01:40:25Z | - |
dc.date.available | 2022-10-31T01:40:25Z | - |
dc.date.issued | 2022-11 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.issn | 0219-1032 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/58922 | - |
dc.description.abstract | As a potential candidate to generate an everlasting cell source to treat various diseases, embryonic stem cells are regarded as a promising therapeutic tool in the regenerative medicine field. Cohesin, a multi-functional complex that controls various cellular activities, plays roles not only in organizing chromosome dynamics but also in controlling transcriptional activities related to self-renewal and differentiation of stem cells. Here, we report a novel role of the alpha-kleisin subunits of cohesin (RAD21 and REC8) in the maintenance of the balance between these two stem-cell processes. By knocking down REC8, RAD21, or the non-kleisin cohesin subunit SMC3 in mouse embryonic stem cells, we show that reduction in cohesin level impairs their self-renewal. Interestingly, the transcriptomic analysis revealed that knocking down each cohesin subunit enables the differentiation of embryonic stem cells into specific lineages. Specifically, embryonic stem cells in which cohesin subunit RAD21 were knocked down differentiated into cells expressing neural alongside germline lineage markers. Thus, we conclude that cohesin appears to control the fate determination of embryonic stem cells. | - |
dc.format.extent | 13 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | The Kleisin Subunits of Cohesin are Involved in the Fate Determination of Embryonic Stem Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.14348/molcells.2022.2042 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.45, no.11, pp 820 - 832 | - |
dc.identifier.kciid | ART002897604 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000867693000001 | - |
dc.identifier.scopusid | 2-s2.0-85142097813 | - |
dc.citation.endPage | 832 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 820 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 45 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | cohesin | - |
dc.subject.keywordAuthor | embryonic stem cells | - |
dc.subject.keywordAuthor | RAD21 | - |
dc.subject.keywordAuthor | REC8 | - |
dc.subject.keywordAuthor | trascriptomic analysis | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | LIMITATIONS | - |
dc.subject.keywordPlus | REGULATOR | - |
dc.subject.keywordPlus | MEIOSIS | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | CULTURE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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