Transcript-specific selective translation by specialized ribosomes bearing genome-encoded heterogeneous rRNAs in V. vulnificus CMCP6
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Younkyung | - |
dc.contributor.author | Joo, Minju | - |
dc.contributor.author | Song, Wooseok | - |
dc.contributor.author | Lee, Minho | - |
dc.contributor.author | Hyeon, Hana | - |
dc.contributor.author | Kim, Hyun-Lee | - |
dc.contributor.author | Yeom, Ji-Hyun | - |
dc.contributor.author | Lee, Kangseok | - |
dc.contributor.author | Shin, Eunkyoung | - |
dc.date.accessioned | 2022-12-09T07:40:27Z | - |
dc.date.available | 2022-12-09T07:40:27Z | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.issn | 1976-3794 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/59538 | - |
dc.description.abstract | Ribosomes composed of genome-encoded heterogeneous rRNAs are implicated in the rapid adaptation of bacterial cells to environmental changes. A previous study showed that ribosomes bearing the most heterogeneous rRNAs expressed from the rrnI operon (I-ribosomes) are implicated in the preferential translation of a subset of mRNAs, including hspA and tpiA, in Vibrio vulnificus CMCP6. In this study, we show that HspA nascent peptides were predominantly bound to I-ribosomes. Specifically, I-ribosomes were enriched more than two-fold in ribosomes that were pulled down by immunoprecipitation of HspA peptides compared with the proportion of I-ribosomes in crude ribosomes and ribosomes pulled down by immunoprecipitation of RNA polymerase subunit β peptides in the wild-type (WT) and rrnI-completed strains. Other methods that utilized the incorporation of an affinity tag in 23S rRNA or chimeric rRNA tethering 16S and 23S rRNAs, which generated specialized functional ribosomes in Escherichia coli, did not result in functional I-ribosomes in V. vulnificus CMCP6. This study provides direct evidence of the preferential translation of hspA mRNA by I-ribosomes. © 2022, Author(s). | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | The Korean Society for Mocrobiology / The Korean Society of Virology | - |
dc.title | Transcript-specific selective translation by specialized ribosomes bearing genome-encoded heterogeneous rRNAs in V. vulnificus CMCP6 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s12275-022-2437-9 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology, v.60, no.12, pp 1162 - 1167 | - |
dc.identifier.kciid | ART002898874 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000887915300005 | - |
dc.identifier.scopusid | 2-s2.0-85142455220 | - |
dc.citation.endPage | 1167 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1162 | - |
dc.citation.title | Journal of Microbiology | - |
dc.citation.volume | 60 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | heterogenous rRNAs | - |
dc.subject.keywordAuthor | pathogenic bacteria | - |
dc.subject.keywordAuthor | ribosome | - |
dc.subject.keywordAuthor | specialized ribosomes | - |
dc.subject.keywordAuthor | V. vulnificus CMCP6 | - |
dc.subject.keywordPlus | STRUCTURALLY DISTINCT | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | CHROMOSOMES | - |
dc.subject.keywordPlus | DNA | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.