Effect of IgG Fc-fusion and KDEL-ER retention signal on prostate-specific antigen expression in plant and its immune in mice
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Chunha | - |
dc.contributor.author | Kim, Kibum | - |
dc.contributor.author | Kang, Yang Joo | - |
dc.contributor.author | Kim, Deuk-Su | - |
dc.contributor.author | Seo, Young-Jin | - |
dc.contributor.author | Park, Se Ra | - |
dc.contributor.author | Kim, Mi Kyung | - |
dc.contributor.author | Lee, Young Koung | - |
dc.contributor.author | Kim, Do-Sun | - |
dc.contributor.author | Ko, Kisung | - |
dc.date.accessioned | 2023-02-14T06:40:25Z | - |
dc.date.available | 2023-02-14T06:40:25Z | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 1863-5466 | - |
dc.identifier.issn | 1863-5474 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/60543 | - |
dc.description.abstract | Prostate-specific antigen (PSA) is a protein highly expressed in cancer cells of the prostate gland. In this study, the PSA recombinant protein was fused to the human immunoglobulin G crystallizable fragment (Fc) with the endoplasmic reticulum (ER) Lys-Asp-Glu-Leu (KDEL) retention signal tag for the plant expression system. Agrobacterium-mediated plant transformation was applied to generate transgenic tobacco plants expressing PSA tagged to KDEL (PSAK), PSA-Fc, and PSA-Fc fused to KDEL (PSA-FcK). Western blot analysis showed that the PSA-FcK protein was the most highly expressed among the three different proteins in the transgenic plant leaf. The PSAK, PSA-Fc, and PSA- FcK proteins were successfully purified from plant leaves. Reduced and non-reduced sodium dodecyl sulfate–polyacrylamide gel electrophoresis revealed that the PSA proteins fused to Fc and FcK were dimerized, whereas the PSA itself was not dimerized. N-glycan analysis demonstrated that the N-glycan profile of the PSA-Fc was mainly the structure with α(1,3)-fucose (Fuc) or β(1,2)-xylose (Xyl) glycan residues, whereas the PSA-Fc tagged with the KDEL ER retention signal had mainly an oligomannose-type structure. ELISA and SPR showed that PSA-FcK had a higher binding activity to Fc gamma receptor I than PSA-Fc and human Fc. In addition, PSA-FcK induced anti-PSA, and thus has the potential to be utilized as a prostate cancer vaccine. © 2022, Korean Society for Plant Biotechnology. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Springer | - |
dc.title | Effect of IgG Fc-fusion and KDEL-ER retention signal on prostate-specific antigen expression in plant and its immune in mice | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s11816-022-00810-9 | - |
dc.identifier.bibliographicCitation | Plant Biotechnology Reports, v.16, no.6, pp 729 - 740 | - |
dc.identifier.kciid | ART002909366 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000897640100002 | - |
dc.identifier.scopusid | 2-s2.0-85143391062 | - |
dc.citation.endPage | 740 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 729 | - |
dc.citation.title | Plant Biotechnology Reports | - |
dc.citation.volume | 16 | - |
dc.type.docType | Article; Early Access | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Fc fusion protein | - |
dc.subject.keywordAuthor | Plant | - |
dc.subject.keywordAuthor | Prostate-specific antigen | - |
dc.subject.keywordAuthor | Therapeutic protein | - |
dc.subject.keywordAuthor | Vaccine candidate | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | PSA | - |
dc.subject.keywordPlus | IMMUNOTHERAPY | - |
dc.subject.keywordPlus | ARABIDOPSIS | - |
dc.subject.keywordPlus | MARKER | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.