Ultrasensitive and amplification-free detection of SARS-CoV-2 RNA using an electrochemical biosensor powered by CRISPR/Cas13a
DC Field | Value | Language |
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dc.contributor.author | Kashefi-Kheyrabadi, Leila | - |
dc.contributor.author | Nguyen, Huynh Vu | - |
dc.contributor.author | Go, Anna | - |
dc.contributor.author | Lee, Min-Ho | - |
dc.date.accessioned | 2023-02-15T06:41:06Z | - |
dc.date.available | 2023-02-15T06:41:06Z | - |
dc.date.issued | 2023-04 | - |
dc.identifier.issn | 1567-5394 | - |
dc.identifier.issn | 1878-562X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/60614 | - |
dc.description.abstract | This study proposed a CRISPR/Cas13a-powered electrochemical multiplexed biosensor for detecting SARS-CoV-2 RNA strands. Current SARS-CoV-2 diagnostic methods, such as reverse transcription PCR (RT-PCR), are primarily based on nucleic acid amplification (NAA) and reverse transcription (RT) processes, which have been linked to significant issues such as cross-contamination and long turnaround times. Using a CRISPR/Cas13a system integrated onto an electrochemical biosensor, we present a multiplexed and NAA-free strategy for detecting SARS-CoV-2 RNA fragments. SARS-CoV-2 S and Orf1ab genes were detected in both synthetic and clinical samples. The CRISPR/Cas13a-powered biosensor achieved low detection limits of 2.5 and 4.5 ag/µL for the S and Orf1ab genes, respectively, successfully meeting the sensitivity requirement. Furthermore, the biosensor's specificity, simplicity, and universality may position it as a potential rival to RT-PCR. © 2023 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Ultrasensitive and amplification-free detection of SARS-CoV-2 RNA using an electrochemical biosensor powered by CRISPR/Cas13a | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bioelechem.2023.108364 | - |
dc.identifier.bibliographicCitation | Bioelectrochemistry, v.150 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000921873600001 | - |
dc.identifier.scopusid | 2-s2.0-85145973054 | - |
dc.citation.title | Bioelectrochemistry | - |
dc.citation.volume | 150 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | CRISPR | - |
dc.subject.keywordAuthor | Electrochemical biosensor | - |
dc.subject.keywordAuthor | Orf1ab gene | - |
dc.subject.keywordAuthor | S gene | - |
dc.subject.keywordAuthor | SARS-CoV-2 RNA | - |
dc.subject.keywordPlus | NUCLEIC-ACID DETECTION | - |
dc.subject.keywordPlus | CRISPR-CAS12A | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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