Highly specific chimeric DNA-RNA-guided genome editing with enhanced CRISPR-Cas12a system
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hanseop | - |
dc.contributor.author | Lee, Wi-jae | - |
dc.contributor.author | Kim, Chan Hyoung | - |
dc.contributor.author | Oh,Yeounsun | - |
dc.contributor.author | Gwon, Lee Wha | - |
dc.contributor.author | Lee, Hyomin | - |
dc.contributor.author | Song, Woojeung | - |
dc.contributor.author | Hur, Junho K. | - |
dc.contributor.author | Lim, Kyung-Seob | - |
dc.contributor.author | Jeong, Kang Jin | - |
dc.contributor.author | Nam,Ki-Hoan | - |
dc.contributor.author | Won,Young-Suk | - |
dc.contributor.author | Lee,Kyeong-Ryoon | - |
dc.contributor.author | Lee, Youngjeon | - |
dc.contributor.author | Kim,Young-Hyun | - |
dc.contributor.author | Huh, Jae-Won | - |
dc.contributor.author | Jun, Bong-Hyun | - |
dc.contributor.author | Lee, Dong-Seok | - |
dc.contributor.author | Lee, Seung Hwan | - |
dc.date.accessioned | 2023-03-08T06:56:09Z | - |
dc.date.available | 2023-03-08T06:56:09Z | - |
dc.date.issued | 2022-06 | - |
dc.identifier.issn | 2162-2531 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/61316 | - |
dc.description.abstract | The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a system is composed of a Cas12a effector that acts as a DNA-cleaving endonuclease and a crispr RNA (crRNA) that guides the effector to the target DNA. It is considered a key molecule for inducing target-specific gene editing in various living systems. Here, we improved the efficiency and specificity of the CRISPR-Cas12a system through protein and crRNA engineering. In particular, to optimize the CRISPR-Cas12a system at the molecular level, we used a chimeric DNA-RNA guide chemically similar to crRNA to maximize target sequence specificity. Compared with the wild-type (wt)-Cas12a system, when using enhanced Cas12a system (en-Cas12a), the efficiency and target specificity improved on average by 2.58 and 2.77 times, respectively. In our study, when the chimeric DNA-RNA-guided en-Cas12a effector was used, the gene-editing efficiency and accuracy were simultaneously increased. These findings could contribute to highly accurate genome editing, such as human gene therapy, in the near future. © 2022 The Authors | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Cell Press | - |
dc.title | Highly specific chimeric DNA-RNA-guided genome editing with enhanced CRISPR-Cas12a system | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.omtn.2022.03.021 | - |
dc.identifier.bibliographicCitation | Molecular Therapy - Nucleic Acids, v.28, pp 353 - 362 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000788852300003 | - |
dc.identifier.scopusid | 2-s2.0-85127870087 | - |
dc.citation.endPage | 362 | - |
dc.citation.startPage | 353 | - |
dc.citation.title | Molecular Therapy - Nucleic Acids | - |
dc.citation.volume | 28 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | chimeric DNA-RNA | - |
dc.subject.keywordAuthor | efficient | - |
dc.subject.keywordAuthor | en-Cas12a | - |
dc.subject.keywordAuthor | gene therapy | - |
dc.subject.keywordAuthor | genome editing | - |
dc.subject.keywordAuthor | highly specific | - |
dc.subject.keywordAuthor | RNA/DNA editing | - |
dc.subject.keywordPlus | R-LOOP COMPLEX | - |
dc.subject.keywordPlus | WIDE SPECIFICITIES | - |
dc.subject.keywordPlus | CRISPR | - |
dc.subject.keywordPlus | CPF1 | - |
dc.subject.keywordPlus | CAS9 | - |
dc.subject.keywordPlus | ENDONUCLEASE | - |
dc.subject.keywordPlus | NUCLEASES | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.