Electrochemical detection of caspase-3 based on a chemically modified M13 phage virus
DC Field | Value | Language |
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dc.contributor.author | Shin, Jae Hwan | - |
dc.contributor.author | Gul, Anam Rana | - |
dc.contributor.author | Hyun, Moon Seop | - |
dc.contributor.author | Choi, Chang-Hyung | - |
dc.contributor.author | Park, Tae Jung | - |
dc.contributor.author | Park, Jong Pil | - |
dc.date.accessioned | 2023-03-08T06:57:07Z | - |
dc.date.available | 2023-03-08T06:57:07Z | - |
dc.date.issued | 2022-06 | - |
dc.identifier.issn | 1567-5394 | - |
dc.identifier.issn | 1878-562X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/61323 | - |
dc.description.abstract | Caspase-3, a cysteine-dependent protease, is considered a reliable molecular biomarker for the diagnosis and prognosis of apoptosis-related diseases. In this study, we demonstrated a phage-based electrochemical biosensor for the evaluation of cell apoptosis by the sensitive and specific detection of caspase-3. Specifically, for screening of affinity peptide-displayed phages, phage display was performed using M13 phage libraries (cyclic forms of peptides), and we identified potential affinity peptide-displayed phage clones with the sequence CPTTMWRYC. After characterization of its binding affinity using enzyme-linked immunosorbent assay, whole phage particles were covalently attached to a gold surface using coupling chemistry (MUA-EDC/NHS). The developed phage sensor was characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscopy (SEM), electrochemical analysis using cyclic voltammetry (CV), and square wave voltammetry (SWV). Under optimal conditions, the affinity peptide-displayed phage sensor showed a good binding affinity (Kd = 0.13 ± 0.56 μM) and limit of detection (0.39 μM) for caspase-3 detection. Furthermore, developed phage sensor could be monitored the response of apoptotic HeLa cells by detecting caspase-3 activity. This work should stimulate the development of efficient alternative caspase-3 detection methods for the diagnosis and prognosis of apoptosis-related diseases. © 2022 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Electrochemical detection of caspase-3 based on a chemically modified M13 phage virus | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bioelechem.2022.108090 | - |
dc.identifier.bibliographicCitation | Bioelectrochemistry, v.145 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000790444600002 | - |
dc.identifier.scopusid | 2-s2.0-85125426709 | - |
dc.citation.title | Bioelectrochemistry | - |
dc.citation.volume | 145 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Caspase-3 | - |
dc.subject.keywordAuthor | Chemical modification | - |
dc.subject.keywordAuthor | Electrochemical phage sensor | - |
dc.subject.keywordPlus | LIGHT-UP PROBE | - |
dc.subject.keywordPlus | SENSITIVE DETECTION | - |
dc.subject.keywordPlus | CELL APOPTOSIS | - |
dc.subject.keywordPlus | COLORIMETRIC DETECTION | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | SURFACE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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