Quantitative determination of bakkenolide D in Petasites japonicus and Farfugium japonicum by HPLC/UV
- Authors
- Quilantang, N.G.; Lee, K.H.; Lee, D.G.; Lee, J.S.; Cho, E.; Kim, H.Y.; Lee, S.
- Issue Date
- Dec-2017
- Publisher
- Korean Society of Pharmacognosy
- Keywords
- Bakkenolide D; Farfugium japonicum; HPLC/UV; Petasites japonicus
- Citation
- Natural Product Sciences, v.23, no.4, pp 270 - 273
- Pages
- 4
- Journal Title
- Natural Product Sciences
- Volume
- 23
- Number
- 4
- Start Page
- 270
- End Page
- 273
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/6142
- DOI
- 10.20307/nps.2017.23.4.270
- ISSN
- 1226-3907
- Abstract
- A quantitative analysis of bakkenolide D in the different parts of Petasites japonicus and Farfugium japonicum was performed by HPLC. A gradient HPLC elution system with a mobile phase consisting of water: acetonitrile solution (20:80 to 0:100 for 45 min) was followed and an INNO C18 column was used for the chromatographic separation. The injection volume, flow rate, and UV detection were 10 µL, 1 mL/min, and 290 nm, respectively. Results show that both species showed the highest amount of bakkenolide D in the roots being 107.203 and 166.103 mg/g for P. japonicas and F. japonicum, respectively. Content analysis on the different parts of both plants displayed remarkably lower values which ranged from 0.403 - 4.419 and 7.252 - 32.614 mg/g for P. japonicas and F. japonicum, respectively. The results show that the roots of both plants are rich in bakkenolide D showing a promising use in the development of nutraceuticals and industrial application of the compound. © 2017, Korean Society of Pharmacognosy. All rights reserved.
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