Mechanistic insight into human androgen receptor-mediated endocrine-disrupting potentials by a stable bioluminescence resonance energy transfer-based dimerization assay
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, S.-H. | - |
dc.contributor.author | Hong, K.Y. | - |
dc.contributor.author | Seo, H. | - |
dc.contributor.author | Lee, H.-S. | - |
dc.contributor.author | Park, Y. | - |
dc.date.accessioned | 2023-03-08T10:05:20Z | - |
dc.date.available | 2023-03-08T10:05:20Z | - |
dc.date.issued | 2021-11-01 | - |
dc.identifier.issn | 0009-2797 | - |
dc.identifier.issn | 1872-7786 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/62051 | - |
dc.description.abstract | To develop a novel cell-based assay to evaluate the androgenic endocrine-disrupting properties of chemical substances, we established a method to detect ligand-mediated androgen receptor (AR) dimerization in stably transfected human cell lines using a bioluminescence resonance energy transfer (BRET) system. Using stably transfected human embryonic kidney (HEK-293) cells, the BRET-based AR dimerization assay was optimized as a novel test method and was validated using test chemicals recommended by the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM). The BRET-based AR dimerization assay showed high accuracy, sensitivity, and specificity for the detection of androgenic endocrine-disrupting chemicals (EDCs), and the assay protocol is adequate for practical use. This dimerization assay is based on ligand-mediated hormone receptor dimerization and can provide accurate information about androgenic endocrine-disrupting properties at the cellular level, complementing conventional binding and transactivation assays. © 2021 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier Ireland Ltd | - |
dc.title | Mechanistic insight into human androgen receptor-mediated endocrine-disrupting potentials by a stable bioluminescence resonance energy transfer-based dimerization assay | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.cbi.2021.109655 | - |
dc.identifier.bibliographicCitation | Chemico-Biological Interactions, v.349 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000704506500003 | - |
dc.identifier.scopusid | 2-s2.0-85115765908 | - |
dc.citation.title | Chemico-Biological Interactions | - |
dc.citation.volume | 349 | - |
dc.type.docType | Article | - |
dc.publisher.location | 아일랜드 | - |
dc.subject.keywordAuthor | Androgen receptor (AR) | - |
dc.subject.keywordAuthor | Bioluminescence resonance energy transfer (BRET) | - |
dc.subject.keywordAuthor | Dimerization assay | - |
dc.subject.keywordAuthor | Endocrine-disrupting chemicals (EDCs) | - |
dc.subject.keywordPlus | BINDING ASSAY | - |
dc.subject.keywordPlus | OCCUPANCY | - |
dc.subject.keywordPlus | REPORTER | - |
dc.subject.keywordPlus | SYSTEMS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.