Magnesium hydroxide-incorporated PLGA composite attenuates inflammation and promotes BMP2-induced bone formation in spinal fusion
DC Field | Value | Language |
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dc.contributor.author | Bedair, Tarek M. | - |
dc.contributor.author | Lee, Chang Kyu | - |
dc.contributor.author | Kim, Da-Seul | - |
dc.contributor.author | Baek, Seung-Woon | - |
dc.contributor.author | Bedair, Hanan M. | - |
dc.contributor.author | Joshi, Hari Prasad | - |
dc.contributor.author | Choi, Un Yong | - |
dc.contributor.author | Park, Keun-Hong | - |
dc.contributor.author | Park, Wooram | - |
dc.contributor.author | Han, InBo | - |
dc.contributor.author | Han, Dong Keun | - |
dc.date.accessioned | 2023-03-08T13:45:50Z | - |
dc.date.available | 2023-03-08T13:45:50Z | - |
dc.date.issued | 2020-10 | - |
dc.identifier.issn | 2041-7314 | - |
dc.identifier.issn | 2041-7314 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/63213 | - |
dc.description.abstract | Spinal fusion has become a common surgical technique to join two or more vertebrae to stabilize a damaged spine; however, the rate of pseudarthrosis (failure of fusion) is still high. To minimize pseudarthrosis, bone morphogenetic protein-2 (BMP2) has been approved for use in humans. In this study, we developed a poly(lactide-co-glycolide) (PLGA) composite incorporated with magnesium hydroxide (MH) nanoparticles for the delivery of BMP2. This study aimed to evaluate the effects of released BMP2 from BMP2-immobilized PLGA/MH composite scaffold in an in vitro test and an in vivo mice spinal fusion model. The PLGA/MH composite films were fabricated via solvent casting technique. The surface of the PLGA/MH composite scaffold was modified with polydopamine (PDA) to effectively immobilize BMP2 on the PLGA/MH composite scaffold. Analyzes of the scaffold revealed that using PLGA/MH-PDA improved hydrophilicity, degradation performance, neutralization effects, and increased BMP2 loading efficiency. In addition, releasing BMP2 from the PLGA/MH scaffold significantly promoted the proliferation and osteogenic differentiation of MC3T3-E1 cells. Furthermore, the pH neutralization effect significantly increased in MC3T3-E1 cells cultured on the BMP2-immobilized PLGA/MH scaffold. In our animal study, the PLGA/MH scaffold as a BMP2 carrier attenuates inflammatory responses and promotes BMP2-induced bone formation in posterolateral spinal fusion model. These results collectively demonstrate that the BMP2-immobilized PLGA/MH scaffold offers great potential in effectively inducing bone formation in spinal fusion surgery. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SAGE PUBLICATIONS INC | - |
dc.title | Magnesium hydroxide-incorporated PLGA composite attenuates inflammation and promotes BMP2-induced bone formation in spinal fusion | - |
dc.type | Article | - |
dc.identifier.doi | 10.1177/2041731420967591 | - |
dc.identifier.bibliographicCitation | JOURNAL OF TISSUE ENGINEERING, v.11 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000586576400001 | - |
dc.identifier.scopusid | 2-s2.0-85093948266 | - |
dc.citation.title | JOURNAL OF TISSUE ENGINEERING | - |
dc.citation.volume | 11 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Spinal fusion | - |
dc.subject.keywordAuthor | osteogenesis | - |
dc.subject.keywordAuthor | poly(lactide-co-glycolide) | - |
dc.subject.keywordAuthor | magnesium hydroxide | - |
dc.subject.keywordAuthor | bone morphogenetic protein-2 | - |
dc.subject.keywordPlus | DECELLULARIZED EXTRACELLULAR-MATRIX | - |
dc.subject.keywordPlus | MORPHOGENETIC PROTEIN-2 | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | PARTICLES | - |
dc.subject.keywordPlus | POROSITY | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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