Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment
DC Field | Value | Language |
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dc.contributor.author | Kang, Seung-Hun | - |
dc.contributor.author | Lee, Wi-jae | - |
dc.contributor.author | An, Ju-Hyun | - |
dc.contributor.author | Lee, Jong-Hee | - |
dc.contributor.author | Kim, Young-Hyun | - |
dc.contributor.author | Kim, Hanseop | - |
dc.contributor.author | Oh, Yeounsun | - |
dc.contributor.author | Park, Young-Ho | - |
dc.contributor.author | Jin, Yeung Bae | - |
dc.contributor.author | Jun, Bong-Hyun | - |
dc.contributor.author | Hur, Junho K. | - |
dc.contributor.author | Kim, Sun-Uk | - |
dc.contributor.author | Lee, Seung Hwan | - |
dc.date.accessioned | 2023-03-08T13:58:07Z | - |
dc.date.available | 2023-03-08T13:58:07Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/63348 | - |
dc.description.abstract | CRISPR effectors, which comprise a CRISPR-Cas protein and a guide (g)RNA derived from the bacterial immune system, are widely used for target-specific genome editing. When the gRNA recognizes genomic loci with sequences that are similar to the target, deleterious mutations can occur. Off-target mutations with a frequency below 0.5% remain mostly undetected by current genome-wide off-target detection techniques. Here we report a method to effectively detect extremely small amounts of mutated DNA based on predicted off-target-specific amplification. In this study, we used various genome editors to induce intracellular genome mutations, and the CRISPR amplification method detected off-target mutations at a significantly higher rate (1.6 similar to 984 fold increase) than an existing targeted amplicon sequencing method. In the near future, CRISPR amplification in combination with genome-wide off-target detection methods will allow detection of genome editor-induced off-target mutations with high sensitivity and in a non-biased manner. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41467-020-17418-8 | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, v.11, no.1 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000552423000015 | - |
dc.identifier.scopusid | 2-s2.0-85088154734 | - |
dc.citation.number | 1 | - |
dc.citation.title | NATURE COMMUNICATIONS | - |
dc.citation.volume | 11 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordPlus | NEXT-GENERATION | - |
dc.subject.keywordPlus | WEB TOOL | - |
dc.subject.keywordPlus | NUCLEASES | - |
dc.subject.keywordPlus | CPF1 | - |
dc.subject.keywordPlus | SEQ | - |
dc.subject.keywordPlus | MUTAGENESIS | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | CHOPCHOP | - |
dc.subject.keywordPlus | RARE | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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