Comparative evaluation of hormones and hormone-like molecule in lineage specification of human induced pluripotent stem cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Seon-A | - |
dc.contributor.author | An, Ju-Hyun | - |
dc.contributor.author | Lee, Seung | - |
dc.contributor.author | Lee, Geun-Hui | - |
dc.contributor.author | Yang, Hae-Jun | - |
dc.contributor.author | Jeong, Pil-Soo | - |
dc.contributor.author | Cha, Jae-Jin | - |
dc.contributor.author | Lee, Sanghoon | - |
dc.contributor.author | Park,Young-Ho | - |
dc.contributor.author | Song, Bong-Seok | - |
dc.contributor.author | Sim, Bo-Woong | - |
dc.contributor.author | Kim, Young-Hyun | - |
dc.contributor.author | Kim,Ji-Su | - |
dc.contributor.author | Jin, Yeung Bae | - |
dc.contributor.author | Huh,Jae-Won | - |
dc.contributor.author | Lee, Sang-Rae | - |
dc.contributor.author | Lee, Jong-Hee | - |
dc.contributor.author | Kim, Sun-Uk | - |
dc.date.accessioned | 2023-03-08T15:00:14Z | - |
dc.date.available | 2023-03-08T15:00:14Z | - |
dc.date.issued | 2019-07 | - |
dc.identifier.issn | 2005-3606 | - |
dc.identifier.issn | 2005-5447 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/63730 | - |
dc.description.abstract | Background and Objectives: Proficient differentiation of human pluripotent stem cells (hPSCs) into specific lineages is required for applications in regenerative medicine. A growing amount of evidences had implicated hormones and hormone-like molecules as critical regulators of proliferation and lineage specification during in vivo development. Therefore, a deeper understanding of the hormones and hormone-like molecules involved in cell fate decisions is critical for efficient and controlled differentiation of hPSCs into specific lineages. Thus, we functionally and quantitatively compared the effects of diverse hormones (estradiol 17-β (E2), progesterone (P4), and dexamethasone (DM)) and a hormone-like molecule (retinoic acid (RA)) on the regulation of hematopoietic and neural lineage specification. Methods and Results: We used 10 nM E2, 3 μM P4, 10 nM DM, and 10 nM RA based on their functional in vivo developmental potential. The sex hormone E2 enhanced functional activity of hematopoietic progenitors compared to P4 and DM, whereas RA impaired hematopoietic differentiation. In addition, E2 increased CD34+CD45+ cells with progenitor functions, even in the CD43- population, a well-known hemogenic marker. RA exhibited lineage-biased potential, preferentially committing hPSCs toward the neural lineage while restricting the hematopoietic fate decision. Conclusions: Our findings reveal unique cell fate potentials of E2 and RA treatment and provide valuable differentiation information that is essential for hPSC applications. © 2019 by the Korean Society for Stem Cell Research. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Sungkyunkwan University | - |
dc.title | Comparative evaluation of hormones and hormone-like molecule in lineage specification of human induced pluripotent stem cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.15283/ijsc18137 | - |
dc.identifier.bibliographicCitation | International Journal of Stem Cells, v.12, no.2, pp 240 - 250 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85071477410 | - |
dc.citation.endPage | 250 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 240 | - |
dc.citation.title | International Journal of Stem Cells | - |
dc.citation.volume | 12 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Cell fate decision | - |
dc.subject.keywordAuthor | Estradiol-17β | - |
dc.subject.keywordAuthor | Hematopoietic differentiation | - |
dc.subject.keywordAuthor | Human induced pluripotent stem cells | - |
dc.subject.keywordAuthor | Lineage specification | - |
dc.subject.keywordAuthor | Retinoic acid | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.