Validation of a liquid chromatography-triple quadrupole mass spectrometric method for the determination of 5-nitro-5-hydroxy-indirubin-3-oxime (AGM-130) in human plasma and its application to microdose clinical trial
DC Field | Value | Language |
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dc.contributor.author | Park, Min-Ho | - |
dc.contributor.author | Lee, Yun Young | - |
dc.contributor.author | Cho, Kyung Hee | - |
dc.contributor.author | La, Sookie | - |
dc.contributor.author | Lee, Hee Joo | - |
dc.contributor.author | Yim, Dong-Seok | - |
dc.contributor.author | Ban, Sooho | - |
dc.contributor.author | Park, Moon-Young | - |
dc.contributor.author | Kim, Yong-Chul | - |
dc.contributor.author | Kim, Yoon-Gyoon | - |
dc.contributor.author | Shin, Young G. | - |
dc.date.accessioned | 2023-03-08T17:51:10Z | - |
dc.date.available | 2023-03-08T17:51:10Z | - |
dc.date.issued | 2016-03 | - |
dc.identifier.issn | 0269-3879 | - |
dc.identifier.issn | 1099-0801 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/64301 | - |
dc.description.abstract | A liquid chromatography-triple quadrupole mass spectrometric (LC-MS/MS) method was developed and validated for the determination of 5-nitro-5-hydroxy-indirubin-3-oxime (AGM-130) in human plasma to support a microdose clinical trial. The method consisted of a liquid-liquid extraction for sample preparation and LC-MS/MS analysis in the positive ion mode using TurboIonSpray(TM) for analysis. d(3)-AGM-130 was used as the internal standard. A linear regression (weighted 1/concentration) was used to fit calibration curves over the concentration range of 10-2000 pg/mL for AGM-130. There were no endogenous interference components in the blank human plasma tested. The accuracy at the lower limit of quantitation was 96.6% with a precision (coefficient of variation, CV) of 4.4%. For quality control samples at 30, 160 and 1600 pg/mL, the between run CV was 5.0 %. Between-run accuracy ranged from 98.1 to 101.0%. AGM-130 was stable in 50% acetonitrile for 168 h at 4 degrees C and 6 h at room temperature. AGM-130 was also stable in human plasma at room temperature for 6 h and through three freeze-thaw cycles. The variability of selected samples for the incurred sample reanalysis was 12.7% when compared with the original sample concentrations. This validated LC-MS/MS method for determination of AGM-130 was used to support a phase 0 microdose clinical trial. Copyright (c) 2015 John Wiley & Sons, Ltd. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Validation of a liquid chromatography-triple quadrupole mass spectrometric method for the determination of 5-nitro-5-hydroxy-indirubin-3-oxime (AGM-130) in human plasma and its application to microdose clinical trial | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/bmc.3551 | - |
dc.identifier.bibliographicCitation | BIOMEDICAL CHROMATOGRAPHY, v.30, no.3, pp 323 - 329 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000369735700005 | - |
dc.identifier.scopusid | 2-s2.0-84956596691 | - |
dc.citation.endPage | 329 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 323 | - |
dc.citation.title | BIOMEDICAL CHROMATOGRAPHY | - |
dc.citation.volume | 30 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | AGM-130 | - |
dc.subject.keywordAuthor | cyclin-dependent kinase (CDK) inhibitor | - |
dc.subject.keywordAuthor | liquid-liquid extraction | - |
dc.subject.keywordAuthor | LC-MS | - |
dc.subject.keywordAuthor | MS | - |
dc.subject.keywordAuthor | microdose clinical trial | - |
dc.subject.keywordPlus | CYCLIN-DEPENDENT KINASES | - |
dc.subject.keywordPlus | CELL-CYCLE | - |
dc.subject.keywordPlus | CANCER-CELLS | - |
dc.subject.keywordPlus | CDK2 | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | B1 | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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