Fertilisation of cryopreserved sperm and unfertilised quail ovum by intracytoplasmic sperm injection
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Kyung Soo | - |
dc.contributor.author | Park, Tae Sub | - |
dc.contributor.author | Rengaraj, Deivendran | - |
dc.contributor.author | Lee, Hyung Chul | - |
dc.contributor.author | Lee, Hong Jo | - |
dc.contributor.author | Choi, Hee Jung | - |
dc.contributor.author | Mizushima, Shusei | - |
dc.contributor.author | Ono, Tamao | - |
dc.contributor.author | Han, Jae Yong | - |
dc.date.accessioned | 2023-03-08T18:20:05Z | - |
dc.date.available | 2023-03-08T18:20:05Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1031-3613 | - |
dc.identifier.issn | 1448-5990 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/64398 | - |
dc.description.abstract | Intracytoplasmic sperm injection (ICSI) is an important technique in animal biotechnology for animal cloning and conservation of genetic resources, but has been a challenge for avian species. In the present study, we investigated the ability of cryopreserved quail spermatozoa to achieve fertilisation and embryo development. Female quail were killed 70-120min after previous oviposition to collect unfertilised oocytes from the oviduct. Fresh or cryopreserved-thawed spermatozoa were injected into the cytoplasm of unfertilised oocytes, and the manipulated oocytes were incubated in quail surrogate eggshells. Injection of fresh spermatozoa supplemented with inositol 1,4,5-trisphosphate (IP3) resulted in a significantly increased rate of embryo development compared with injection of fresh spermatozoa alone (90% vs 13%, respectively). Although >80% of embryos stopped cell division and development before Hamburger and Hamilton (HH) Stage 3, approximately 15% of embryos from the fresh sperm injection developed to past HH Stage 4, and one embryo survived up to HH Stage 39 (11 days of incubation). In the case of cryopreserved spermatozoa, the embryo development rate was 30% after ICSI, and this increased significantly to 74% with IP3 supplementation. In conclusion, cryopreserved spermatozoa combined with ICSI followed by surrogate eggshell culture can develop quail embryos. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | CSIRO PUBLISHING | - |
dc.title | Fertilisation of cryopreserved sperm and unfertilised quail ovum by intracytoplasmic sperm injection | - |
dc.type | Article | - |
dc.identifier.doi | 10.1071/RD15126 | - |
dc.identifier.bibliographicCitation | REPRODUCTION FERTILITY AND DEVELOPMENT, v.28, no.12, pp 1974 - 1981 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000386048900011 | - |
dc.identifier.scopusid | 2-s2.0-84991481633 | - |
dc.citation.endPage | 1981 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1974 | - |
dc.citation.title | REPRODUCTION FERTILITY AND DEVELOPMENT | - |
dc.citation.volume | 28 | - |
dc.type.docType | Article | - |
dc.publisher.location | 호주 | - |
dc.subject.keywordAuthor | surrogate eggshell | - |
dc.subject.keywordPlus | FLUORESCENT PROTEIN EXPRESSION | - |
dc.subject.keywordPlus | POULTRY SEMEN | - |
dc.subject.keywordPlus | COTURNIX-JAPONICA | - |
dc.subject.keywordPlus | FOWL SPERMATOZOA | - |
dc.subject.keywordPlus | JAPANESE-QUAIL | - |
dc.subject.keywordPlus | ICSI | - |
dc.subject.keywordPlus | CALCIUM | - |
dc.subject.keywordPlus | OOCYTES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | REPRODUCTION | - |
dc.relation.journalResearchArea | Developmental Biology | - |
dc.relation.journalResearchArea | Reproductive Biology | - |
dc.relation.journalResearchArea | Zoology | - |
dc.relation.journalWebOfScienceCategory | Developmental Biology | - |
dc.relation.journalWebOfScienceCategory | Reproductive Biology | - |
dc.relation.journalWebOfScienceCategory | Zoology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.