Proteomic identification of cryostress in epididymal spermatozoa
DC Field | Value | Language |
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dc.contributor.author | Yoon, Sung Jae | - |
dc.contributor.author | Rahman, Md Saidur | - |
dc.contributor.author | Kwon, Woo Sung | - |
dc.contributor.author | Ryu, Do Yeal | - |
dc.contributor.author | Park, Yoo Jin | - |
dc.contributor.author | Pang, Myung Geol | - |
dc.date.available | 2019-03-08T12:00:03Z | - |
dc.date.issued | 2016-11 | - |
dc.identifier.issn | 2049-1891 | - |
dc.identifier.issn | 2049-1891 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/6439 | - |
dc.description.abstract | Background: Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation, thereby reducing sperm quality. We assessed the motility (%), motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence staining. Moreover, we identified proteins associated with cryostress using a proteomic approach and performed western blotting to validate two-dimensional electrophoresis (2-DE) results using two commercial antibodies. Results: Cryopreservation reduced viability (%), motility (%), straight-line velocity (VSL), average path velocity (VAP), amplitude of lateral head displacement (ALH), and capacitated spermatozoa, whereas straightness (STR) and the acrosome reaction increased after cryopreservation (P < 0.05). Nine proteins were differentially expressed (two proteins decreased and seven increased) (> 3 fold, P < 0.05) before and after cryopreservation. The proteins differentially expressed following cryopreservation are putatively related to several signaling pathways, including the ephrinR-actin pathway, the ROS metabolism pathway, actin cytoskeleton assembly, actin cytoskeleton regulation, and the guanylate cyclase pathway. Conclusion: The results of the current study provide information on epididymal sperm proteome dynamics and possible protein markers of cryo-stress during cryopreservation. This information will further the basic understanding of cryopreservation and aid future studies aiming to identify the mechanism of cryostress responses. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.title | Proteomic identification of cryostress in epididymal spermatozoa | - |
dc.type | Article | - |
dc.identifier.doi | 10.1186/s40104-016-0128-2 | - |
dc.identifier.bibliographicCitation | JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY, v.7, no.1 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000388246800001 | - |
dc.identifier.scopusid | 2-s2.0-84997270831 | - |
dc.citation.number | 1 | - |
dc.citation.title | JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY | - |
dc.citation.volume | 7 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Cryopreservation | - |
dc.subject.keywordAuthor | Cryostress | - |
dc.subject.keywordAuthor | Protein | - |
dc.subject.keywordAuthor | Spermatozoa | - |
dc.subject.keywordPlus | MANGANESE SUPEROXIDE-DISMUTASE | - |
dc.subject.keywordPlus | HUMAN SPERM CAPACITATION | - |
dc.subject.keywordPlus | 2 DIFFERENT EXTENDERS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | BOVINE SPERMATOZOA | - |
dc.subject.keywordPlus | MALE-FERTILITY | - |
dc.subject.keywordPlus | TRIOSEPHOSPHATE ISOMERASE | - |
dc.subject.keywordPlus | ACTIN POLYMERIZATION | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | ACROSOME REACTION | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Dairy & Animal Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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