In Vivo Measurement of Site-Specific Peritoneal Solute Transport Using a Fiber-Optic-based Fluorescence Photobleaching Technique
DC Field | Value | Language |
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dc.contributor.author | Lee, Donghee | - |
dc.contributor.author | Kim, Jeong Chul | - |
dc.contributor.author | Shin, Eunkyoung | - |
dc.contributor.author | Ju, Kyung Don | - |
dc.contributor.author | Oh, Kook-Hwan | - |
dc.contributor.author | Kim, Hee Chan | - |
dc.contributor.author | Kang, Eungtaek | - |
dc.contributor.author | Kim, Jung Kyung | - |
dc.date.accessioned | 2023-03-08T19:00:16Z | - |
dc.date.available | 2023-03-08T19:00:16Z | - |
dc.date.issued | 2015-06 | - |
dc.identifier.issn | 1226-4776 | - |
dc.identifier.issn | 2093-6885 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/64559 | - |
dc.description.abstract | Fluorescence recovery after photobleaching (FRAP) is a well-established method commonly used to measure the diffusion of fluorescent solutes and biomolecules in living cells or tissues. Here a fiber-optic-based FRAP (f-FRAP) system was developed, and validated using macromolecules in water and agarose gels of different concentrations. We applied f-FRAP to measure the site-specific diffusion of fluorescein (NaFluo) in peritoneal membranes (PMs) on the liver, cecum, and kidney of a living rat during peritoneal dialysis. Diffusion of fluorescein in PM varied in a time-dependent manner according to the type of organ (D-PM (on) (Liver)/D-NaFluo = 0.199 +/- 0.085, D-PM (on) (Cecum)/D-NaFluo = 0.292 +/- 0.151, D-PM (on) (Kidney)/D-NaFluo = 0.218 +/- 0.110). The proposed method allows direct quantitative measurement of the three-dimensional diffusion in local PM in vivo, which was previously inaccessible by peritoneal function test methods such as peritoneal equilibration test (PET) and standardized PM assessment (SPA). f-FRAP is promising for local and dynamic assessments of peritoneal pathophysiology and the mass transport properties of PMs, presumed to be affected by variation of tissue structures over different organs and functional changes of the PM with years of peritoneal dialysis. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | OPTICAL SOC KOREA | - |
dc.title | In Vivo Measurement of Site-Specific Peritoneal Solute Transport Using a Fiber-Optic-based Fluorescence Photobleaching Technique | - |
dc.type | Article | - |
dc.identifier.doi | 10.3807/JOSK.2015.19.3.228 | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE OPTICAL SOCIETY OF KOREA, v.19, no.3, pp 228 - 236 | - |
dc.identifier.kciid | ART001999688 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000357350200003 | - |
dc.identifier.scopusid | 2-s2.0-84938078181 | - |
dc.citation.endPage | 236 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 228 | - |
dc.citation.title | JOURNAL OF THE OPTICAL SOCIETY OF KOREA | - |
dc.citation.volume | 19 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Fluorescence recovery after photobleaching (FRAP) | - |
dc.subject.keywordAuthor | Peritoneal membrane | - |
dc.subject.keywordAuthor | Diffusion | - |
dc.subject.keywordAuthor | Fiberoptics | - |
dc.subject.keywordPlus | MACROMOLECULE DIFFUSION | - |
dc.subject.keywordPlus | ULTRAFILTRATION FAILURE | - |
dc.subject.keywordPlus | MEMBRANE-FUNCTION | - |
dc.subject.keywordPlus | RECOVERY | - |
dc.subject.keywordPlus | DIALYSIS | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | KINETICS | - |
dc.subject.keywordPlus | MOBILITY | - |
dc.relation.journalResearchArea | Optics | - |
dc.relation.journalWebOfScienceCategory | Optics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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