Ylpex5 mutation partially suppresses the defective hyphal growth of a Yarrowia lipolytica ceramide synthase mutant, Yllac1, by recovering lipid raft polarization and vacuole morphogenesis
DC Field | Value | Language |
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dc.contributor.author | Bal, J. | - |
dc.contributor.author | Lee, H.-J. | - |
dc.contributor.author | Cheon, S.A. | - |
dc.contributor.author | Lee, K.J. | - |
dc.contributor.author | Oh, D.-B. | - |
dc.contributor.author | Kim, J.-Y. | - |
dc.date.accessioned | 2023-03-08T21:35:46Z | - |
dc.date.available | 2023-03-08T21:35:46Z | - |
dc.date.issued | 2013-01 | - |
dc.identifier.issn | 1087-1845 | - |
dc.identifier.issn | 1096-0937 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/64883 | - |
dc.description.abstract | Sphingolipids are involved in cell differentiation and morphogenesis in eukaryotic cells. In this study, YlLac1p, a ceramide synthase required for glucosylceramide (GlcCer) synthesis, was found to be essential for hyphal growth in Yarrowia lipolytica. Y. lipolytica GlcCer was shown to be composed of a C16:0 fatty acid, which is hydroxylated at C2, and a C18:2 long chain base, which is unsaturated at both C4 and C8 and methylated at C9. Domain swapping analysis revealed that the entire TRAM/Lag1/CLN8 (TLC) domain, not the Lag1 motif, is crucial for the function of YlLac1p. YlDes1p, the C4 desaturase of the ceramide synthesized by YlLac1p, was also required for Y. lipolytica morphogenesis. Both Yllac1Δ and Yldes1Δ mutants neither polarize lipid rafts nor form normal vacuoles. Interestingly, mutation in YlPEX5, which encode a peroxisomal targeting signal receptor, partially suppressed the defective hyphal growth of Yllac1Δ. The Yllac1Δ Ylpex5Δ mutant restored the ability to polarize lipid rafts and to form normal vacuoles, although it could not synthesize GlcCer. Taken together, our results suggest that GlcCer or GlcCer derivatives may be involved in hyphal morphogenesis in Y. lipolytica, at least in part, by affecting polarization of lipid rafts and vacuole morphogenesis. © 2012 Elsevier Inc. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.title | Ylpex5 mutation partially suppresses the defective hyphal growth of a Yarrowia lipolytica ceramide synthase mutant, Yllac1, by recovering lipid raft polarization and vacuole morphogenesis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.fgb.2012.11.003 | - |
dc.identifier.bibliographicCitation | Fungal Genetics and Biology, v.50, no.1, pp 1 - 10 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84871375187 | - |
dc.citation.endPage | 10 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | Fungal Genetics and Biology | - |
dc.citation.volume | 50 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Ceramide synthase | - |
dc.subject.keywordAuthor | Glucosylceramide | - |
dc.subject.keywordAuthor | Hyphal growth | - |
dc.subject.keywordAuthor | YlLac1 | - |
dc.subject.keywordAuthor | YlPEX5 | - |
dc.subject.keywordPlus | fungal protein | - |
dc.subject.keywordPlus | glucosylceramide | - |
dc.subject.keywordPlus | longevity assurance gene 1 protein | - |
dc.subject.keywordPlus | pex5 protein | - |
dc.subject.keywordPlus | protein CLN8 | - |
dc.subject.keywordPlus | protein Des1 | - |
dc.subject.keywordPlus | protein Lac1 | - |
dc.subject.keywordPlus | structural protein | - |
dc.subject.keywordPlus | translocating chain associating membrane protein | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | cell maturation | - |
dc.subject.keywordPlus | cell polarity | - |
dc.subject.keywordPlus | cell vacuole | - |
dc.subject.keywordPlus | Des1 gene | - |
dc.subject.keywordPlus | fungal development | - |
dc.subject.keywordPlus | fungal gene | - |
dc.subject.keywordPlus | fungus growth | - |
dc.subject.keywordPlus | fungus hyphae | - |
dc.subject.keywordPlus | fungus mutant | - |
dc.subject.keywordPlus | gene deletion | - |
dc.subject.keywordPlus | hydroxylation | - |
dc.subject.keywordPlus | Lac1 gene | - |
dc.subject.keywordPlus | lipid raft | - |
dc.subject.keywordPlus | lipogenesis | - |
dc.subject.keywordPlus | methylation | - |
dc.subject.keywordPlus | mutational analysis | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | pex5 gene | - |
dc.subject.keywordPlus | phenotype | - |
dc.subject.keywordPlus | priority journal | - |
dc.subject.keywordPlus | protein domain | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | protein function | - |
dc.subject.keywordPlus | protein motif | - |
dc.subject.keywordPlus | signal transduction | - |
dc.subject.keywordPlus | Yarrowia lipolytica | - |
dc.subject.keywordPlus | Hyphae | - |
dc.subject.keywordPlus | Membrane Microdomains | - |
dc.subject.keywordPlus | Membrane Proteins | - |
dc.subject.keywordPlus | Microscopy | - |
dc.subject.keywordPlus | Oxidoreductases | - |
dc.subject.keywordPlus | Suppression, Genetic | - |
dc.subject.keywordPlus | Vacuoles | - |
dc.subject.keywordPlus | Yarrowia | - |
dc.subject.keywordPlus | Eukaryota | - |
dc.subject.keywordPlus | Yarrowia lipolytica | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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