역전사중합효소 연쇄반응-효소면역측정법을 활용한 장내바이러스 감염 진단Application of a Diagnostic Method Using Reverse Transcription-PCR ELISA for the Diagnosis of Enteroviral Infections
- Authors
- 박귀성; 이강범; 백경아; 정은혜; 박성민; 조영채; 송재형; 안광숙; 천두성
- Issue Date
- Dec-2009
- Publisher
- KOREAN SOC LABORATORY MEDICINE
- Keywords
- Enterovirus; Aseptic meningitis; Conventional RT-PCR; RT PCR-ELISA
- Citation
- KOREAN JOURNAL OF LABORATORY MEDICINE, v.29, no.6, pp 594 - 600
- Pages
- 7
- Journal Title
- KOREAN JOURNAL OF LABORATORY MEDICINE
- Volume
- 29
- Number
- 6
- Start Page
- 594
- End Page
- 600
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/65132
- DOI
- 10.3343/kjlm.2009.29.6.594
- ISSN
- 1598-6535
- Abstract
- 배경 : 장바이러스(enterovirus)는 무균성 수막염의 주요 병 원체이다. 무균성 수막염이 의심되는 경우, 장바이러스에 대한 정확한 진단이 필수적이지만 기존의 세포배양을 통한 바이러스 의 분리법은 많은 시간과 노력이 필요하다. 그러므로 최근에는 장바이러스 진단을 위하여 신속하고 민감한 분자생물학적 방법 이 사용되고 있다. 방법 : 장바이러스의 유전자 중 잘 보존된 부분인 5’non-coding region에 대한 소식자를 이용하여 reverse transcription (RT)-PCR ELISA 키트를 제작하였다. 제작된 RT-PCR ELISA 키트는 RT-PCR법과 비교되었는데, 바이러스 분리주와 무균성 수막염이 의심되는 환자 검체에 대하여 민감도와 특이도를 측 정하였다. 결과 : RT-PCR ELISA법의 검출한계는 세포배양을 통한 바 이러스 분리법과 RT-PCR법보다 10-100배 높았으며, 또한 무 균성 수막염 환자의 임상 시료를 대상으로 한 RT-PCR ELISA 법의 검출률이 RT-PCR법 보다 7% 높게 나타났다(P=0.016). 결론 : 본 연구에서 활용된 RT-PCR ELISA법은 높은 민감 도와 특이도를 보여 장바이러스 진단법으로 이용할 것을 제안 한다.
Background : Enteroviruses are known as major pathogen for aseptic meningitis. Although rapid diagnosis for enteroviruses is very essential to exclude bacterial infections in patients with meningitis, classical diagnostic method based on virus isolation is not practicable for timely treatment of patients due to its laborious and time-consuming procedure. Recently molecular methodologies as alternatives are routinely used for rapid and sensitive diagnosis for enteroviruses infections. Methods: Reverse transcription (RT)-PCR ELISA kit for targeting 5'non-coding region (NCR) with highly conserved genetic identity among all genotypes of enteroviruses was introduced in this investigation. RT-PCR ELISA was evaluated about sensitivity and specificity through virus isolation using clinical specimens from patients suspected of enteroviral infections and enteroviral isolates comparing with conventional RT-PCR identifying them. Results : The detection limit of the RT-PCR ELISA was up to 10-100 folds higher than virus isolation using cell culture and conventional RT-PCR. On comparison between above two methods, the detection rate of RT-PCR ELISA for clinical specimens from patients with aseptic meningitis was 7% higher than that of conventional RT-PCR targeting 5'NCR (P=0.016). Conclusions : Our results suggest that RT-PCR ELISA developed in this study could be an alternative diagnostic method for the detection of enteroviral genome with high sensitivity and specificity. (Korean J Lab Med 2009;29:594-600)
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Biotechnology & Natural Resource > School of Food Science and Technology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/65132)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.