Evaluation of hydrophobic films for high sensitive and self aligning micro array protein chip

Abstract

Well plate based ELISA is one of the most widely used immunoassay techniques for the detection of a target protein. However, this method has some technical limits such as poor signal sensitivity, poor detection limit, large sample consumption, high evaporation rate, and low signal/noise ratio. In this paper, we fabricated a high sensitive pre-patterned micro array chip by using semiconductor process where chips have hydrophilic and hydrophobic patterns on a glass substrate. And we could acquire the high resolution scanning images on the microarray chip with hydrophobic patterning which has low protein adsorption. © 2009 CBMS.