Human neural stem cells overexpressing glial cell line-derived neurotrophic factor in experimental cerebral hemorrhage
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, H. J. | - |
dc.contributor.author | Park, I. H. | - |
dc.contributor.author | Kim, H. J. | - |
dc.contributor.author | Kim, S. U. | - |
dc.date.accessioned | 2023-03-08T23:38:11Z | - |
dc.date.available | 2023-03-08T23:38:11Z | - |
dc.date.issued | 2009-09 | - |
dc.identifier.issn | 0969-7128 | - |
dc.identifier.issn | 1476-5462 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/65198 | - |
dc.description.abstract | Recent studies have reported that glial cell line-derived growth factor (GDNF) has neurotrophic effects on the central nervous system, and the neural stem cells (NSCs) engrafted in animal models of stroke survive and ameliorate the neurological deficits. In this study, a stable human NSC line overexpressing GDNF (F3.GDNF) was transplanted next to the intracerebral hemorrhage (ICH) lesion site and a possible therapeutic effect was investigated. F3.GDNF human NSC line was transplanted into the cortex overlying the striatal ICH lesion. ICH was induced in adult mice by the unilateral injection of bacterial collagenase into the striatum. The animals were evaluated for 8 weeks with rotarod and limb placement tests. Transplanted NSCs were detected by beta-gal immunostaining with double labeling of neurofilament, microtubule associated protein-2, glial fibrillary acidic protein or human nuclear matrix antigen (HuNuMA). F3.GDNF human NSCs produced a four times higher amount of GDNF over parental F3 cells in vitro, induced behavioral improvement in ICH mice after brain transplantation and two- to threefold increase in cell survival of transplanted NSCs at 2 and 8 weeks post-transplantation. In F3.GDNF-grafted ICH brain, a significant increase in the antiapoptotic protein and cell survival signal molecules, and a marked reduction in proapoptotic proteins were found as compared with control group. Brain transplantation of human NSCs overexpressing GDNF in ICH animals provided functional recovery in ICH animals, and survival and differentiation of grafted human NSCs. These results indicate that the F3.GDNF human NSCs should be of a great value as a cellular source for the cellular therapy in animal models of human neurological disorders including ICH. Gene Therapy (2009) 16, 1066-1076; doi: 10.1038/gt.2009.51; published online 25 June 2009 | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Human neural stem cells overexpressing glial cell line-derived neurotrophic factor in experimental cerebral hemorrhage | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/gt.2009.51 | - |
dc.identifier.bibliographicCitation | GENE THERAPY, v.16, no.9, pp 1066 - 1076 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000269673200002 | - |
dc.identifier.scopusid | 2-s2.0-70249116026 | - |
dc.citation.endPage | 1076 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1066 | - |
dc.citation.title | GENE THERAPY | - |
dc.citation.volume | 16 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | brain transplantation | - |
dc.subject.keywordAuthor | GDNF | - |
dc.subject.keywordAuthor | human neural stem cell | - |
dc.subject.keywordAuthor | intracerebral hemorrhage | - |
dc.subject.keywordAuthor | neuroprotection | - |
dc.subject.keywordAuthor | stroke | - |
dc.subject.keywordPlus | FUNCTIONAL RECOVERY | - |
dc.subject.keywordPlus | ARTERY OCCLUSION | - |
dc.subject.keywordPlus | HUMAN NEURONS | - |
dc.subject.keywordPlus | GDNF | - |
dc.subject.keywordPlus | ISCHEMIA | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | RATS | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | PROTECTS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.