Inhibitory effect of conditioned medium of silk fibroin-treated osteoblasts in osteoclast differentiation
DC Field | Value | Language |
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dc.contributor.author | Yeo, J.-H. | - |
dc.contributor.author | Park, K.-H. | - |
dc.contributor.author | Ju, W.-C. | - |
dc.contributor.author | Lee, J. | - |
dc.contributor.author | Lee, K.-G. | - |
dc.contributor.author | Woo, S.-O. | - |
dc.contributor.author | Han, S.-M. | - |
dc.contributor.author | Kweon, H. | - |
dc.contributor.author | Kim, S.-S. | - |
dc.contributor.author | Cho, Y. | - |
dc.date.accessioned | 2023-03-09T00:20:43Z | - |
dc.date.available | 2023-03-09T00:20:43Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1226-3311 | - |
dc.identifier.issn | 2288-5978 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/65346 | - |
dc.description.abstract | In this study, we investigated the indirect effect of silk-fibroin on osteoclastic differentiation of RAW264.7 cells. The conditioned medium were collected from MC3T3-E1 osbeoblasts treated with 0.001 mg/mL~0.1 mg/mL silk fibroin for 6 days, mixed in 1:1 ratio with osteoclast medium, and then added into RAW264.7 cells with receptor activator of nuclear factor kappa B ligand (RANKL), a differentiation inducer for 3 days. Of osteoclastic cytokines in the conditioned medium, the protein expression of osteoprotegerin (OPG) with silk-fibroin was not significantly different. However, the protein expression of interleukin (IL)-1β was specifically lower in a dose dependent manner. In RAW264.7 cells, the conditioned medium with silk-fibroin inhibited RANKL induced osteoclastic differentiation as total number of multinucleated tartrate-resistant alkaline phosphatase (TRAP)-positive osteoclasts in a dose dependent manner. Taken together, we demonstrated that the conditioned medium of silk-fibroin treated osteoblasts inhibits RANKL induced differentiation of osteoclasts with inhibiting selective expression of IL-1β. | - |
dc.format.extent | 6 | - |
dc.language | 한국어 | - |
dc.language.iso | KOR | - |
dc.publisher | Korean Society of Food Science and Nutrition | - |
dc.title | Inhibitory effect of conditioned medium of silk fibroin-treated osteoblasts in osteoclast differentiation | - |
dc.type | Article | - |
dc.identifier.doi | 10.3746/jkfn.2008.37.8.992 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Society of Food Science and Nutrition, v.37, no.8, pp 992 - 997 | - |
dc.identifier.kciid | ART001271076 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84969849365 | - |
dc.citation.endPage | 997 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 992 | - |
dc.citation.title | Journal of the Korean Society of Food Science and Nutrition | - |
dc.citation.volume | 37 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Conditioned medium | - |
dc.subject.keywordAuthor | Interleukin-1β | - |
dc.subject.keywordAuthor | Osteoclastic differentiation | - |
dc.subject.keywordAuthor | Osteoprotegerin | - |
dc.subject.keywordAuthor | Silk-fibroin | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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