카드뮴폭로에 대한 생물학적 지표 Competitive ELISA에 의한 Metallothionein 정량
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박정덕 | - |
dc.contributor.author | 이우석 | - |
dc.contributor.author | 공윤 | - |
dc.contributor.author | 홍연표 | - |
dc.contributor.author | 장임원 | - |
dc.date.accessioned | 2023-05-30T06:41:03Z | - |
dc.date.available | 2023-05-30T06:41:03Z | - |
dc.date.issued | 1996-09 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66619 | - |
dc.description.abstract | Metallothionein (MT) is a cadmium binding protein that played major roles in protective mechanism for cadmium toxicity. In the present study, competitive ELISA was estab- lished to assay the MT expression utilizing monospecific antibody which was generated against MT-I. A total of 10 Sprague-Dawley rats was injected with CdCla for two weeks to induce MT. The cytosolic fraction of rat liver was obtained by differential centrifuga- tion. Two major MT isozymes (MT-I & MT-II) at ca. 10 kDa were purified by Sephadex G-75 gel filtration followed by DEAE-Trisacryl-M anion exchange column chromatogra- phy, respectively. Two rabbits were immunized 4 times consecutively with the conjugate of purified MT-I. The sera were collected by heart puncture. When immunoblot. was car ried out, the immunized rabbit sera (anti-MT-1) exhibited specific immunoreactive band at MT-I while showed any cross reactions neither with MT-II nor with other cytosolic pro teins. By chequerboard titration using the monospecific antibody, the competitive ELISA was established. The dose-dependent relationship was observed between anti-MT-I anti- body and the amount of MT in biological samples (r=0.9980). These results suggested strongly that competitive ELISA is a simple, rapid and reproducible method for screening cadmium exposure. | - |
dc.format.extent | 14 | - |
dc.publisher | 대한산업의학회 | - |
dc.title | 카드뮴폭로에 대한 생물학적 지표 Competitive ELISA에 의한 Metallothionein 정량 | - |
dc.title.alternative | Biological Index for Cadmium Exposure : Assay of Metallothionein by Competitive ELISA | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | 대한산업의학회지, v.8, no.2, pp 258 - 271 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 271 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 258 | - |
dc.citation.title | 대한산업의학회지 | - |
dc.citation.volume | 8 | - |
dc.identifier.url | https://www.dbpia.co.kr/journal/articleDetail?nodeId=NODE01155866 | - |
dc.subject.keywordAuthor | Metallothionein (MT) | - |
dc.subject.keywordAuthor | Cadmium Toxicity | - |
dc.subject.keywordAuthor | Competitive ELISA, anti-MT-I | - |
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