시호의 메탄올 추출물이 일차배양한 뇌, DRG 및 간세포에 미치는 영향

Abstract

Effects of the methanol extract of Bupleuri Radix on primary cultured. chicken embryonic brain cells, dorsal root ganglia (DRG) and rat hepatocytes were studied. The methanol extract of Bupleuri Radix at the concentration ranging from 10 ㎍/㎖ to 100 ㎍/㎖ significantly recovered the cytotoxicity of rat hepatocytes induced by the treatment of galactosamine; at the concentration of 100 ㎍/㎖, values of GOT and GPT in the culture medium were reduced by the 60% and 75%, respectively of those in the absence of the methanol extract of Bupleuri Radix. The addition of the methanol extract of Bupleuri Radix, into chicken embryonic brain cells which were cultured with a deficient medium significantly increased the number of cells promoting the neurite outgrowth. However, the methanol extract of Bupleuri Radix showed no effect on the activities of PDHC and acetylcholinesterase in primary cultured brain cells.