Expression of a recombinant chimeric protein of human colorectal cancer antigen GA733-2 and Fc fragment of antibody using a replicating vector based on Beet curly top virus in infiltrated Nicotiana benthamiana leaves
DC Field | Value | Language |
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dc.contributor.author | Kim, Kyung Il | - |
dc.contributor.author | Chung, Ha Young | - |
dc.contributor.author | Yoo, Ki Hyun | - |
dc.contributor.author | Park, Jong-Hwa | - |
dc.contributor.author | Lee, Hyun Ho | - |
dc.contributor.author | Soek, Yeon Ju | - |
dc.contributor.author | Ko, Ki Sung | - |
dc.contributor.author | Kang, Hyung Sik | - |
dc.contributor.author | Lee, Kyung Jin | - |
dc.contributor.author | Oh, Doo-Byoung | - |
dc.contributor.author | Joung, Young Hee | - |
dc.contributor.author | Chung, In Sik | - |
dc.date.accessioned | 2023-06-08T02:40:33Z | - |
dc.date.available | 2023-06-08T02:40:33Z | - |
dc.date.issued | 2012-07 | - |
dc.identifier.issn | 1863-5466 | - |
dc.identifier.issn | 1863-5474 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66688 | - |
dc.description.abstract | We describe expression and characterization of recombinant human colorectal cancer antigen GA733-2 fused to Fc fragment of antibody (GA733-2-Fc) using a replicating vector based on Beet curly top virus in infiltrated Nicotiana benthamiana leaves. Recombinant GA733-2-Fc/KDEL with a molecular mass of similar to 68 kDa was transiently expressed. The level of expression of GA733-2-Fc with ER retention signal KDEL (GA733-2-Fc/KDEL) in the expression vector system was 0.96% of total soluble proteins. Recombinant GA733-2-Fc/KDEL was purified using an affinity chromatography. Mice immunized with recombinant GA733-2-Fc/KDEL mounted a strong GA733-2-Fc/KDEL-specific serum antibody response. Vaccination of plant-derived recombinant GA733-2-Fc/KDEL regressed tumor volumes in BALB/c mice. The population of activated-T and NK-T cells increased notably in lymph node, spleen, and tumor-infiltrating lymphocytes derived from the tumor-regressed mice. Taken together, recombinant GA733-2-Fc/KDEL expressed in plants can be used as an effective experimental immunogen for research in cancer vaccine development. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER | - |
dc.title | Expression of a recombinant chimeric protein of human colorectal cancer antigen GA733-2 and Fc fragment of antibody using a replicating vector based on Beet curly top virus in infiltrated Nicotiana benthamiana leaves | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s11816-012-0218-3 | - |
dc.identifier.bibliographicCitation | PLANT BIOTECHNOLOGY REPORTS, v.6, no.3, pp 233 - 242 | - |
dc.identifier.kciid | ART001710577 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000306440000006 | - |
dc.identifier.scopusid | 2-s2.0-84864133620 | - |
dc.citation.endPage | 242 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 233 | - |
dc.citation.title | PLANT BIOTECHNOLOGY REPORTS | - |
dc.citation.volume | 6 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Colorectal cancer | - |
dc.subject.keywordAuthor | GA733-2 | - |
dc.subject.keywordAuthor | Nicotiana benthamiana | - |
dc.subject.keywordAuthor | BCTV (Beet curly top virus) | - |
dc.subject.keywordAuthor | Vaccination | - |
dc.subject.keywordPlus | CARCINOMA-ASSOCIATED ANTIGEN | - |
dc.subject.keywordPlus | ALPHA-GALACTOSYLCERAMIDE | - |
dc.subject.keywordPlus | NKT CELLS | - |
dc.subject.keywordPlus | T-CELLS | - |
dc.subject.keywordPlus | MOLECULE | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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