Immunoaffinity Purification and Receptor Assay for Polymerized Human Serum Albumin of Pre-S2 Peptide on Hepatitis B Virus
DC Field | Value | Language |
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dc.contributor.author | Yun, Hye Young | - |
dc.contributor.author | Han, Moon H | - |
dc.contributor.author | Hahm, Kyung-Soo | - |
dc.date.accessioned | 2023-06-09T08:43:23Z | - |
dc.date.available | 2023-06-09T08:43:23Z | - |
dc.date.issued | 1988 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66718 | - |
dc.description.abstract | Recombinant pre-S2 peptide was purified to apparent homogeneity from E. coli by immunoaffinity chromatography using monoclonal antibody and studied for its binding characteristics with monoclonal antibody and pHSA (polymerized human serum albumin). A simple and sensitive assay method for detecting the activity of pHSA receptor on pre-S2 peptide of hepatitis B virus surface antigen (HBsAg) has been developed using pre-S2-β-galactosidase fusion protein. The assay can be used for determining pre-S2 or factors neutralizing pHSA receptor activity. The sensitivity of the assay is about 10 to 100 pmol of pre-S2. | - |
dc.description.abstract | 대장균내에서 과량 발현된 재조합 프리-S2 펩티드를 면역친화력 크로마토그래피에 의해 분리 정제하였으며, 이 펩티드에 대한 중합 인혈청 알부민(pHSA)의 결합을 연구하였다. 프리-S2 부위의 pHSA 수용체 활성을 검출하는 방법을 프리-S2-β-갈락토시다제 융합단백질을 이용하여 개발하였으며, 민감도는 10-100 pmol의 프리-S2에 해당되었다. 또한 이 방법은 프리-S2의 농도 결정 뿐 아니라 pHSA수용체 활성을 중화시킬 수 있는 인자들을 측정하는데 이용될 수 있다. | - |
dc.format.extent | 7 | - |
dc.publisher | 한국생화학회 | - |
dc.title | Immunoaffinity Purification and Receptor Assay for Polymerized Human Serum Albumin of Pre-S2 Peptide on Hepatitis B Virus | - |
dc.title.alternative | B형 간염 바이러스 프리-S2 펩티드의 분리 정제 및 중합 인혈청 알부민 수용체 검출법 | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | Korean Biochemical Journal, v.21, no.4, pp 412 - 418 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 418 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 412 | - |
dc.citation.title | Korean Biochemical Journal | - |
dc.citation.volume | 21 | - |
dc.identifier.url | https://kiss.kstudy.com/Detail/Ar?key=117364 | - |
dc.publisher.location | 대한민국 | - |
dc.description.journalRegisteredClass | domestic | - |
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