Mitochondrial alterations in human gastric carcinoma cell line
- Authors
- Kim, Hyoung Kyu; Park, Won Sun; Kang, Sung Hyun; Warda, Mohamad; Kim, Nari; Ko, Jae-Hong; Prince, Abd El-bary; Han, Jin
- Issue Date
- Aug-2007
- Publisher
- American Physiological Society
- Keywords
- Biomarker; Cancer; Two-dimensional gel electrophoresis proteomics
- Citation
- American Journal of Physiology - Cell Physiology, v.293, no.2, pp C761 - C771
- Journal Title
- American Journal of Physiology - Cell Physiology
- Volume
- 293
- Number
- 2
- Start Page
- C761
- End Page
- C771
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66772
- DOI
- 10.1152/ajpcell.00043.2007
- ISSN
- 0363-6143
1522-1563
- Abstract
- We compared mitochondrial function, morphology, and proteome in the rat normal gastric cell line RGM-1 and the human gastric cancer cell line AGS. Total numbers and cross-sectional sizes of mitochondria were smaller in AGS cells. Mitochondria in AGS cells were deformed and consumed less oxygen. Confocal microscopy indicated that the mitochondrial inner membrane potential was hyperpolarized and the mitochondrial Ca2+ concentration was elevated in AGS cells. Interestingly, two-dimensional electrophoresis proteomics on the mitochondria-enriched fraction revealed high expression of four mitochondrial proteins in AGS cells: ubiquinol-cytochrome c reductase, mitochondrial short-chain enoyl-coenzyme A hydratase-1, heat shock protein 60, and mitochondria elongation factor Tu. The results provide clues as to the mechanism of the mitochondrial changes in cancer at the protein level and may serve as potential cancer biomarkers in mitochondria. Copyright © 2007 the American Physiological Society.
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