Voltage-dependent slowly activating anion current regulated by temperature and extracellular pH in mouse B cells
DC Field | Value | Language |
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dc.contributor.author | Nam, Joo Hyun | - |
dc.contributor.author | Zheng, Hai Feng | - |
dc.contributor.author | Earm, Ki Hyun | - |
dc.contributor.author | Ko, Jae Hong | - |
dc.contributor.author | Lee, Ik-Jae | - |
dc.contributor.author | Kang, Tong Mook | - |
dc.contributor.author | Kim, Tae Jin | - |
dc.contributor.author | Earm, Yung E | - |
dc.contributor.author | Kim, Sung Joon | - |
dc.date.accessioned | 2023-06-13T02:41:30Z | - |
dc.date.available | 2023-06-13T02:41:30Z | - |
dc.date.issued | 2006-09 | - |
dc.identifier.issn | 0031-6768 | - |
dc.identifier.issn | 1432-2013 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66779 | - |
dc.description.abstract | Voltage-dependent, outwardly rectifying anion channels have been described in various cells including lymphocytes. In this study, we found that murine B cells express the voltage-dependent slowly activating anion channels (VSACs). Using a whole-cell configuration, IVSAC in Bal-17 was induced by a sustained depolarization (>0 mV) which was remarkably facilitated at 35°C (Q10=23 at 30 mV of clamp voltage). Substitution of extracellular Cl- with gluconate shifted the reversal potential to the right (35.7 mV). Gd3+ (IC50=0.11 μM) significantly attenuated IVSAC, but DIDS partially blocked IVSAC. In addition, extracellular acidification suppressed IVSAC whereas alkalinization facilitated the channel activation. IVSAC was decreased by 90% at pH 6.35 and increased by 180% at pH 8.0. In cell-attached and inside-out patch clamps, depolarization slowly activated the anion channels of large conductance (∼270 pS) with multiple levels of subconductances. The single channel currents were also blocked by Gd3+ and acidic pH. Furthermore, I VSAC was also observed in WEHI-231 (an immature B cell line) and freshly isolated splenic B cells of mice. In summary, murine B cells express unique voltage-dependent anion channels that show a strong sensitivity to both temperature and extracellular pH. Further investigation is required to understand the physiological roles of VSAC and its molecular identity. © Springer-Verlag 2006. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Springer Verlag | - |
dc.title | Voltage-dependent slowly activating anion current regulated by temperature and extracellular pH in mouse B cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s00424-006-0084-3 | - |
dc.identifier.bibliographicCitation | Pflugers Archiv European Journal of Physiology, v.452, no.6, pp 707 - 717 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000241229600007 | - |
dc.identifier.scopusid | 2-s2.0-33748356900 | - |
dc.citation.endPage | 717 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 707 | - |
dc.citation.title | Pflugers Archiv European Journal of Physiology | - |
dc.citation.volume | 452 | - |
dc.type.docType | Article | - |
dc.publisher.location | 독일 | - |
dc.subject.keywordAuthor | Anion channel | - |
dc.subject.keywordAuthor | Voltage-dependent channel | - |
dc.subject.keywordAuthor | Lymphocyte | - |
dc.subject.keywordAuthor | Temperature | - |
dc.subject.keywordAuthor | pH | - |
dc.subject.keywordAuthor | B cell | - |
dc.subject.keywordAuthor | Mouse | - |
dc.subject.keywordPlus | NADPH OXIDASE | - |
dc.subject.keywordPlus | K+ CHANNELS | - |
dc.subject.keywordPlus | ION CHANNELS | - |
dc.subject.keywordPlus | DEPOLARIZATION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | VOLUME | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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