Endothelin-1 inhibits inward rectifier K+ channels in rabbit coronary arterial smooth muscle cells through protein kinase C
DC Field | Value | Language |
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dc.contributor.author | Park, Won Sun | - |
dc.contributor.author | Han, Jin | - |
dc.contributor.author | Kim, Nari | - |
dc.contributor.author | Youm, Jae Boum | - |
dc.contributor.author | Joo, Hyun | - |
dc.contributor.author | Kim, Hyung Kyu | - |
dc.contributor.author | Ko, Jae-Hong | - |
dc.contributor.author | Earm, Yung E. | - |
dc.date.accessioned | 2023-06-13T02:42:09Z | - |
dc.date.available | 2023-06-13T02:42:09Z | - |
dc.date.issued | 2005-11 | - |
dc.identifier.issn | 0160-2446 | - |
dc.identifier.issn | 1533-4023 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66786 | - |
dc.description.abstract | We studied inward rectifier K+ (Kir) channels in smooth muscle cells isolated from rabbit coronary arteries. In cells from small-(<100 μm, SCASMC) and medium-diameter (100 ∼ 200 μm, MCASMC) coronary arteries, Kir currents were clearly identified (11.2 ± 0.6 and 4.2 ± 0.6 pA pF-1 at -140 mV in SCASMC and MCASMC, respectively) that were inhibited by Ba2+ (50 μM). By contrast, a very low Kir current density (1.6 ± 0.4 pA pF-1) was detected in cells from large-diameter coronary arteries (>200 μm, LCASMC). The presence of Kir2.1 protein was confirmed in SCASMC in a Western blot assay. Endothelin-1 (ET-1) inhibited Kir currents in a dose-dependent manner. The inhibition of Kir currents by ET-1 was abolished by pretreatment with the protein kinase C (PKC) inhibitor staurosporine (100 nM) or GF 109203X (1 μM). The PKC activators phorbol 12,13-dibutyrate (PDBu) and 1-oleoyl-2-acetyl-sn-glycerol (OAG) reduced Kir currents. The ETA-receptor inhibitor BQ-123 prevented the ET-1-induced inhibition of Kir currents. The amplitudes of the ATP-dependent K+ (KATP), Ca2+-activated K+ (BKCa), and voltage-dependent K+ (KV) currents, and effects of ET-1 on these channels did not differ between SCASMC and LCASMC. From these results, we conclude that Kir channels are expressed at a higher density in SCASMC than in larger arteries and that the Kir channel activity is negatively regulated by the stimulation of ETA-receptors via the PKC pathway. Copyright © 2005 by Lippincott Williams & Wilkins. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Lippincott Williams & Wilkins Ltd. | - |
dc.title | Endothelin-1 inhibits inward rectifier K+ channels in rabbit coronary arterial smooth muscle cells through protein kinase C | - |
dc.type | Article | - |
dc.identifier.doi | 10.1097/01.fjc.0000182846.08357.ed | - |
dc.identifier.bibliographicCitation | Journal of Cardiovascular Pharmacology, v.46, no.5, pp 681 - 689 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000235808400016 | - |
dc.identifier.scopusid | 2-s2.0-27144496593 | - |
dc.citation.endPage | 689 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 681 | - |
dc.citation.title | Journal of Cardiovascular Pharmacology | - |
dc.citation.volume | 46 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | coronary arteryendothelin-1inward rectifier K+ channelKir2.1protein kinase C | - |
dc.subject.keywordPlus | POTASSIUM CHANNELS | - |
dc.subject.keywordPlus | ANGIOTENSIN-II | - |
dc.subject.keywordPlus | CATION CHANNELS | - |
dc.subject.keywordPlus | CURRENTS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | MYOCYTES | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | TONE | - |
dc.relation.journalResearchArea | Cardiac & Cardiovascular SystemsPharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Cardiac & Cardiovascular SystemsPharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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