Higher expression of TRPM7 channels in murine mature B lymphocytes than immature cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Jin Kyoung | - |
dc.contributor.author | Ko, Jae Hong | - |
dc.contributor.author | Nam, Joo Hyun | - |
dc.contributor.author | Woo, Ji Eun | - |
dc.contributor.author | Min, Kyeong Min | - |
dc.contributor.author | Earm, Yung E | - |
dc.contributor.author | Kim, Sung Joon | - |
dc.date.accessioned | 2023-06-13T04:40:36Z | - |
dc.date.available | 2023-06-13T04:40:36Z | - |
dc.date.issued | 2005-04 | - |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.issn | 2093-3827 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66794 | - |
dc.description.abstract | TRPM7, a cation channel protein permeable to various metal ions such as Mg2+, is ubiquitously expressed in variety of cells including lymphocytes. The activity of TRPM7 is tightly regulated by intracellular Mg2+, thus named Mg2+-inhibited cation (MIC) current, and its expression is known to be critical for the viability and proliferation of B lymphocytes. In this study, the level of MIC current was compared between immature (WEHI-231) and mature (Bal-17) B lymphocytes. In both cell types, an intracellular dialysis with Mg2+-free solution (140 mM CsCl) induced an outwardly-rectifying MIC current. The peak amplitude of MIC current and the permeability to divalent cation (Mn2+) were several fold higher in Bal-17 than WEHI-231. Also, the level of mRNAs for TRPM7, a molecular correspondence of the MIC channel, was significantly higher in Bal-17 cells. The amplitude of MIC was further increased, and the relation between current and voltage became linear under divalent cation-free conditions, demonstrating typical properties of the TRPM7. The stimulation of B cell receptors (BCR) by ligation with antibodies did not change the amplitude of MIC current. Also, increase of extracellular [Mg2+]c to enhance the Mg2+ influx did not affect the BCR ligation-induced death of WEHI-231 cells. Although the level of TRPM7 was not directly related with the cell death of immature B cells, the remarkable difference of TRPM7 might indicate a fundamental change in the permeability to divalent cations during the development of B cells. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 대한약리학회 | - |
dc.title | Higher expression of TRPM7 channels in murine mature B lymphocytes than immature cells | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | The Korean Journal of Physiology & Pharmacology, v.9, no.2, pp 69 - 75 | - |
dc.identifier.kciid | ART000965683 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-18944382565 | - |
dc.citation.endPage | 75 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 69 | - |
dc.citation.title | The Korean Journal of Physiology & Pharmacology | - |
dc.citation.volume | 9 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | B lymphocyte | - |
dc.subject.keywordAuthor | Bal-17 | - |
dc.subject.keywordAuthor | Cell death | - |
dc.subject.keywordAuthor | Nonselective cation channel | - |
dc.subject.keywordAuthor | TRPM7 | - |
dc.subject.keywordAuthor | WEHI-231 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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