Staurosporine inhibits voltage-dependent K+ current through a PKC-independent mechanism in isolated coronary arterial smooth muscle cells
DC Field | Value | Language |
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dc.contributor.author | Park, Won Sun | - |
dc.contributor.author | Son, Youn Kyoung | - |
dc.contributor.author | Han, Jin | - |
dc.contributor.author | Kim, Nari | - |
dc.contributor.author | Ko, Jae-Hong | - |
dc.contributor.author | Bae, Young Min | - |
dc.contributor.author | Earm, Yung E. | - |
dc.date.accessioned | 2023-06-13T05:40:26Z | - |
dc.date.available | 2023-06-13T05:40:26Z | - |
dc.date.issued | 2005-03 | - |
dc.identifier.issn | 0160-2446 | - |
dc.identifier.issn | 1533-4023 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66797 | - |
dc.description.abstract | We examined the effects of the protein kinase C (PKC) inhibitor staurosporine (ST) on voltage-dependent K+ (KV) channels in rabbit coronary arterial smooth muscle cells. ST inhibited the KV current in a dose-dependent manner with a Kd value of 1.3 μM. The inhibition of the KV current by ST was voltage-dependent between -30 and +10 mV. The additive inhibition of the KV current by ST was voltage-dependent throughout the activation voltage range. The rate constants of association and dissociation of ST were 0.63 μM-1 s-1 and 0.92 s-1, respectively. ST produced use-dependent inhibition of the KV current. ST shifted the activation curve to more positive potentials but did not have any significant effect on the voltage dependence of the inactivation curve. ST did not have any significant effects on other types of K+ channel. Another PKC inhibitor, chelerythrine, and PKA inhibitor peptide (PKA-IP) had little effect on the KV current. These results suggest that ST interacts with KV channels that are in the closed state and that ST inhibits KV channels in the open state in a manner that is phosphorylation-independent and voltage-, time-, and use-dependent. Copyright © 2005 by Lippincott Williams & Wilkins. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Lippincott Williams & Wilkins Ltd. | - |
dc.title | Staurosporine inhibits voltage-dependent K+ current through a PKC-independent mechanism in isolated coronary arterial smooth muscle cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1097/01.fjc.0000154370.57789.fe | - |
dc.identifier.bibliographicCitation | Journal of Cardiovascular Pharmacology, v.45, no.3, pp 260 - 269 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000227407100011 | - |
dc.identifier.scopusid | 2-s2.0-14744273136 | - |
dc.citation.endPage | 269 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 260 | - |
dc.citation.title | Journal of Cardiovascular Pharmacology | - |
dc.citation.volume | 45 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | coronary arterial smooth musclevoltage-dependent K+ channelprotein kinase Cstaurosporineelectrophysiologyopen block | - |
dc.subject.keywordPlus | PROTEIN-KINASE-C | - |
dc.subject.keywordPlus | HAMSTER OVARY CELLS | - |
dc.subject.keywordPlus | POTASSIUM CHANNEL | - |
dc.subject.keywordPlus | DIRECT BLOCK | - |
dc.subject.keywordPlus | BISINDOLYLMALEIMIDE | - |
dc.subject.keywordPlus | MYOCYTES | - |
dc.subject.keywordPlus | HEART | - |
dc.subject.keywordPlus | PORE | - |
dc.subject.keywordPlus | CHELERYTHRINE | - |
dc.subject.keywordPlus | POTENT | - |
dc.relation.journalResearchArea | Cardiac & Cardiovascular SystemsPharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Cardiac & Cardiovascular SystemsPharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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