Involvement of ERK and p38 MAP kinase in AAPH-induced COX-2 expression in HaCaT cells
DC Field | Value | Language |
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dc.contributor.author | Cui, Yong | - |
dc.contributor.author | Kim, Dong-Seok | - |
dc.contributor.author | Park, Seo-Hyoung | - |
dc.contributor.author | Yoon, Jin-A | - |
dc.contributor.author | Kim, Soon-Kyum | - |
dc.contributor.author | Kwon, Sun-Bang | - |
dc.contributor.author | Park, Kyoung-Chan | - |
dc.date.accessioned | 2023-06-16T05:43:40Z | - |
dc.date.available | 2023-06-16T05:43:40Z | - |
dc.date.issued | 2004-04 | - |
dc.identifier.issn | 0009-3084 | - |
dc.identifier.issn | 1873-2941 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66858 | - |
dc.description.abstract | Cyclooxygenase-2 (COX-2) appears to play an important role in inflammation and carcinogenesis, and 2,2'-azobis (2-amidino-propane) dihydrochloride (AAPH) is a hydrophilic azo compound known to generate free radicals. Because reactive oxygen species (ROS) are known to elevate COX-2 expression, we evaluated the effect of AAPH on the expression of COX-2 in a human keratinocyte cell line, HaCaT When cells were exposed to AAPH, marked COX-2 induction was observed. To clarify the signaling mechanism involved, we next investigated the effects of AAPH upon three major subfamilies of the mitogen-activated protein kinases (MAPKs). AAPH caused an increase in the phosphorylation of extracellular signal-regulated kinase (ERK), p38 and c-Jun NH2-terminal kinase (JNK). Furthermore, we found that PD98059, an ERK pathway inhibitor, and S13203580, a p38 MAPK inhibitor, diminished AAPH-induced COX-2 expression and PGE(2) production, whereas JNK inhibitor did not suppress COX-2 expression or PGE, production by AAPH. These findings suggest that the ERK and p38 MAPK pathways, but not the JNK pathway, are involved in AAPH-induced inflammatory progression. In addition, we found that both the water-soluble Vitamin E derivative. Trolox, and the green tea constituent, (-)-epigallocatechin gallate (EGCG), diminished AAPH-induced COX-2 expression and p38 activation. (C) 2003 Elsevier Ireland Ltd. All rights reserved. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.title | Involvement of ERK and p38 MAP kinase in AAPH-induced COX-2 expression in HaCaT cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.chemphyslip.2003.11.004 | - |
dc.identifier.bibliographicCitation | CHEMISTRY AND PHYSICS OF LIPIDS, v.129, no.1, pp 43 - 52 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000220344100004 | - |
dc.identifier.scopusid | 2-s2.0-1442274906 | - |
dc.citation.endPage | 52 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 43 | - |
dc.citation.title | CHEMISTRY AND PHYSICS OF LIPIDS | - |
dc.citation.volume | 129 | - |
dc.type.docType | Article | - |
dc.publisher.location | 아일랜드 | - |
dc.subject.keywordAuthor | ERK | - |
dc.subject.keywordAuthor | p38 MAPK | - |
dc.subject.keywordAuthor | AAPH | - |
dc.subject.keywordAuthor | COX-2 | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 GENE-EXPRESSION | - |
dc.subject.keywordPlus | LIPID-PEROXIDATION | - |
dc.subject.keywordPlus | HUMAN KERATINOCYTES | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | ANTIOXIDANTS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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