Sphingosine-1-phosphate promotes mouse melanocyte survival via ERK and Akt activation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Dong-Seok | - |
dc.contributor.author | Hwang, Eui-Soo | - |
dc.contributor.author | Lee, Jai-Eun | - |
dc.contributor.author | Kim, Sook-Young | - |
dc.contributor.author | Park, Kyoung-Chan | - |
dc.date.accessioned | 2023-06-16T05:43:58Z | - |
dc.date.available | 2023-06-16T05:43:58Z | - |
dc.date.issued | 2003-10 | - |
dc.identifier.issn | 0898-6568 | - |
dc.identifier.issn | 1873-3913 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66862 | - |
dc.description.abstract | In this study, we investigated the signalling pathways induced by ultraviolet B (UVB) and the effects of sphingosine-1-phosphate on UVB-induced apoptosis of mouse melanocytes, Mel-Ab, and observed the cytoprotective effects of sphingosine-1-phosphate on UVB-induced apoptosis. Since sphingosine-1-phosphate is a well-known mitogenic agent, we thought it possible that the mitogenic effect of sphingosine-1-phosphate might contribute to cell survival. However, we found that sphingosine-1-phosphate significantly inhibits DNA synthesis. We next examined the regulation of the three major subfamilies of mitogen-activated protein (MAP) kinases and of the Akt pathway by sphingosine-1-phosphate against UVB-induced apoptosis. UVB irradiation resulted in the remarkable and sustained activation of c-Jun N-terminal kinase (INK), while p38 MAP kinase was only transiently activated. The basal level of extracellular signal-regulated protein kinase (ERK) phosphorylation decreased 30 min after UVB irradiation, whereas the basal level of Akt phosphorylation was unaffected by UVB. We also found that sphingosine-1-phosphate potently stimulates the phosphorylation of both ERK and Akt, which are involved in the cell survival-signalling cascade. Furthermore, the specific inhibition of the ERK and Akt pathways by PD98059 and LY294002, respectively, restored the cytoprotective effect induced by sphingosine-1-phosphate. On the other hand, the p38 inhibitor SB203580 additively enhanced the cytoprotective effect on sphingosine-1-phosphate. Based on these results, we conclude that the activation of p38 MAP kinase plays an important role in UVB-induced apoptosis, and that sphingosine-1-phosphate probably exert its cytoprotective effect in Mel-Ab cells through ERK and Akt activation. (C) 2003 Elsevier Science Inc. All rights reserved. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.title | Sphingosine-1-phosphate promotes mouse melanocyte survival via ERK and Akt activation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/S0898-6568(03)00055-x | - |
dc.identifier.bibliographicCitation | CELLULAR SIGNALLING, v.15, no.10, pp 919 - 926 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000184594600004 | - |
dc.identifier.scopusid | 2-s2.0-0038447079 | - |
dc.citation.endPage | 926 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 919 | - |
dc.citation.title | CELLULAR SIGNALLING | - |
dc.citation.volume | 15 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | sphingosine-1-phosphate | - |
dc.subject.keywordAuthor | UV | - |
dc.subject.keywordAuthor | cytoprotection | - |
dc.subject.keywordAuthor | proliferation | - |
dc.subject.keywordAuthor | ERK | - |
dc.subject.keywordAuthor | Akt | - |
dc.subject.keywordPlus | N-TERMINAL KINASE | - |
dc.subject.keywordPlus | SPHINGOSINE 1-PHOSPHATE | - |
dc.subject.keywordPlus | SIGNALING PATHWAYS | - |
dc.subject.keywordPlus | HUMAN KERATINOCYTES | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | CANCER CELLS | - |
dc.subject.keywordPlus | MAP KINASE | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.