Alteration of the activated responses in platelet-activating factor-stimulated neutrophils by protein kinase inhibitors
DC Field | Value | Language |
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dc.contributor.author | Lee, Kang-Kun | - |
dc.contributor.author | Ko, Ji-Young | - |
dc.contributor.author | Ham, Dong-Suk | - |
dc.contributor.author | Shin, Yong-Kyoo | - |
dc.contributor.author | Lee, Chung-Soo | - |
dc.date.accessioned | 2023-06-26T06:40:22Z | - |
dc.date.available | 2023-06-26T06:40:22Z | - |
dc.date.issued | 1996-04 | - |
dc.identifier.issn | 0377-9459 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66999 | - |
dc.description.abstract | Roles of protein kinase C and protein tyrosine kinase in the activation of neutrophil respiratory burst, degranulation and elevation of cytosolic Ca2+ in platelet-activating factor (PAF)-stimulated neutrophils were investigated. Superoxide and H2O2 production and myeloperoxidase and acid phosphatase release in PAF-stimulated neutrophils were inhibited by protein kinase C inhibitors, staurosporine and H-7 and protein tyrosine kinase inhibitors, genistein and tyrphostin. The PAF-induced elevation of [Ca2+](i) in neutrophils was inhibited by staurosporine, genistein and methyl-2,5-dihydroxycinnamate. Staurosporine inhibited both intracellular Ca2+ release and Mn2+ influx in PAF-stimulated neutrophils. Genistein and methyl-2,5-dihydroxycinnamate inhibited Mn2+ influx induced by PAF, whereas their effects on intracellular Ca2+ release were not detected. In neutrophils preactivated by PMA, the stimulatory effect of PAF on the elevation of [Ca2+](i) was reduced. Protein kinase C and protein tyrosine kinase may be involved in respiratory burst, lysosomal enzyme release and Ca2+ mobilization in PAF-stimulated neutrophils. The elevation of [Ca2+](i) appears to be accomplished by intracellular Ca2+ release and Ca2+ influx which are differently regulated by protein kinases. Preactivation of protein kinase C appears to attenuate the stimulatory action of PAF on intracellular Ca2+ mobilization. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Eui-Hak Publishing and Printing Co. | - |
dc.title | Alteration of the activated responses in platelet-activating factor-stimulated neutrophils by protein kinase inhibitors | - |
dc.type | Article | - |
dc.identifier.doi | 10.4196/kjpp.1996.32.1.103 | - |
dc.identifier.bibliographicCitation | Korean Journal of Pharmacology, v.32, no.1, pp 103 - 112 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-0029794257 | - |
dc.citation.endPage | 112 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 103 | - |
dc.citation.title | Korean Journal of Pharmacology | - |
dc.citation.volume | 32 | - |
dc.identifier.url | https://www.kjpp.net/journal/view.html?uid=2593&vmd=Full&jname=Pharmacology | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Protein kinase inhibitor | - |
dc.subject.keywordAuthor | Platelet-activating factor | - |
dc.subject.keywordAuthor | Neutrophil responses | - |
dc.description.journalRegisteredClass | scopus | - |
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