Role of calcium and protein kinase C in platelet activating factor- induced activation of peritoneal macrophages
- Authors
- Lee, Chung-Soo; Kim, Young-Jun; Shin, Yong-Kyoo; Lee, Kwang-Soo
- Issue Date
- Jun-1993
- Publisher
- Eui-Hak Publishing and Printing Co.
- Keywords
- Ca2+mobilization; Degranulation; Superoxide generation; Peritoneal macrophage; Platelet activating factor; Human neutrophil
- Citation
- Korean Journal of Pharmacology, v.29, no.1, pp 107 - 120
- Pages
- 14
- Journal Title
- Korean Journal of Pharmacology
- Volume
- 29
- Number
- 1
- Start Page
- 107
- End Page
- 120
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/67005
- DOI
- 10.4196/kjpp.1993.29.1.107
- ISSN
- 0377-9459
- Abstract
- Particulate or soluble stimuli appear to stimulate phagocytic cell's response by the change of Ca2+ mobilization and by the activation of protein kinase C. In contrast, it is reported that activation of protein kinase C could attenuate agonist-stimulated elevation of Ca2+i in neutrophils. PAF elicited an increase of Ca2+i in peritoneal macrophages in a dose dependent fashion and Ca2+ extrusion was accompanied. PAF-induced elevation of Ca2+i was not affected by TMB-8, verapamil and TTX. TEA stimulated PAF-induced mobilization of Ca2+i and delayed lowering of Ca2+i. Five mM EGTA almost completely inhibited PAF-induced mobilization of Ca2+i. After the addition of PAF, membrane permeability was markedly increased up to 5 min and then slowly increased. PAF-induced LDH release was slightly decreased by EGTA plus TMB-8. PAF-stimulated superoxide generation was inhibited by EGTA, TMB-8 and verapamil but not affected by TTX and TEA. PAF-induced elevation of Ca2+i, increased membrane permeability and superoxide generation were inhibited by IQSP, chlorpromazine and propranolol. PAF-induced LDH release was significantly inhibited by chlorpromazine and minimally decreased by propranolol. After the pretreatment with PMA, the stimulatory effect of PAF on the elevation of Ca2+i and LDH release in macrophages was significantly decreased. These results suggest that PAF may exert the stimulatory action on peritoneal macrophages of mouse by the elevation of Ca2+i and by the activation of protein kinase C. Preactivation of protein kinase C appears to attenuate the stimulatory action of PAF on macrophage response.
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